Detailed information of TA system TA022576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7788..8428
• Replicon: plasmid pMT-pPCP
• Accession: NC_010158
• Organism: Yersinia pestis Angola

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: YPANGOLA_RS00055
• Protein ID: WP_002225538.1
• Coordinates: 8087..8428 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: YPANGOLA_RS00050
• Protein ID: WP_002213446.1
• Coordinates: 7788..8087 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YPANGOLA_RS00025	2925..3119	+	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-
YPANGOLA_RS00030	4355..4780	+	426	WP_002218512.1	pesticin immunity protein	-
YPANGOLA_RS00035	4815..5888	-	1074	WP_002218509.1	pesticin	-
YPANGOLA_RS00040	6115..6420	+	306	WP_002221218.1	hypothetical protein	-
YPANGOLA_RS00045	6663..7601	+	939	WP_002231174.1	omptin family plasminogen activator Pla	-
YPANGOLA_RS00050	7788..8087	-	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
YPANGOLA_RS00055	8087..8428	-	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YPANGOLA_RS00065	9695..10717	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
YPANGOLA_RS00070	10714..11496	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
YPANGOLA_RS00075	12533..12727	+	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / pla / ymt	1..114570	114570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T22576 WP_002225538.1 NC_010158:c8428-8087 [Yersinia pestis Angola]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T22576 NC_010158:c8428-8087 [Yersinia pestis Angola]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT22576 WP_002213446.1 NC_010158:c8087-7788 [Yersinia pestis Angola]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT22576 NC_010158:c8087-7788 [Yersinia pestis Angola]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016