Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 356489..357135 Replicon chromosome
Accession NZ_CP087634
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02297

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag LMH52_RS01645 Protein ID WP_002920560.1
Coordinates 356489..356836 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LMH52_RS01650 Protein ID WP_002920557.1
Coordinates 356836..357135 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH52_RS01635 (LMH52_01635) 352415..353848 + 1434 WP_002920564.1 glycogen synthase GlgA -
LMH52_RS01640 (LMH52_01640) 353866..356313 + 2448 WP_002920561.1 glycogen phosphorylase -
LMH52_RS01645 (LMH52_01645) 356489..356836 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMH52_RS01650 (LMH52_01650) 356836..357135 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LMH52_RS01655 (LMH52_01655) 357198..358706 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LMH52_RS01660 (LMH52_01660) 358911..359240 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LMH52_RS01665 (LMH52_01665) 359291..360121 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LMH52_RS01670 (LMH52_01670) 360171..360929 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T225759 WP_002920560.1 NZ_CP087634:356489-356836 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T225759 NZ_CP121310:913429-913722 [Ensifer adhaerens]
ATGGCGTTTCGGCTTTCCGTCGCGGCAGAAGAAGACATCATCGCGATCGCCGCAGATGGGGTGCGCCTTTTCGGCTCCGC
CCAGGCGCGGCGATATCACGATGAACTCTTCGCCGTATTCGCCTTGATTGCCGCCAACCCGCGCATGGCGCGCGAACGCA
CGGAGCTGTCGCCGCCGATGCGCATCCATCCGTTCAAGGCGCATCTGGTCGTCTACCGGGTCGAGGCGGACGGCGACATC
CTCGTCGTTCGCGTCCGTCATGGGCATGAGGACTGGATCAGCAACGCATTTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT225759 WP_002920557.1 NZ_CP087634:356836-357135 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT225759 NZ_CP121310:913181-913429 [Ensifer adhaerens]
ATGGCGACGATGAACGTATCCTTGCCCGACCCGATGAAGGATTGGGTGGAAGCGCAAACGCGCACGGGCCGTTATGCCAA
CGCCAGCGATTATGTGCGCGACCTCATCCGCAGGGATCAGGAGCGAAATGACAAGGTTGCGGTGATGCAACGCTTTGTCG
ACGACGGTCTGAAGAGCGGCGTCGGGTCCCGCTCCAAGGACGAGCTCTTTTCCGCGGCGCTTACGCGCGCTGAAGTGCCG
CGCGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References