Detailed information of TA system TA225753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53374..53638
• Replicon: plasmid pDD02341-3
• Accession: NZ_CP087632
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: LMH53_RS29095
• Protein ID: WP_001303307.1
• Coordinates: 53486..53638 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	53374..53436 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH53_RS29080 (49476)	49476..50546	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
LMH53_RS29085 (50565)	50565..51773	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (51953)	51953..52013	-	61	NuclAT_1	-	-
- (51953)	51953..52013	-	61	NuclAT_1	-	-
- (51953)	51953..52013	-	61	NuclAT_1	-	-
- (51953)	51953..52013	-	61	NuclAT_1	-	-
LMH53_RS29090 (52080)	52080..52859	-	780	WP_275450201.1	protein FinQ	-
- (53374)	53374..53436	-	63	NuclAT_0	-	Antitoxin
- (53374)	53374..53436	-	63	NuclAT_0	-	Antitoxin
- (53374)	53374..53436	-	63	NuclAT_0	-	Antitoxin
- (53374)	53374..53436	-	63	NuclAT_0	-	Antitoxin
LMH53_RS29095 (53486)	53486..53638	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
LMH53_RS29100 (53710)	53710..53961	-	252	WP_001291968.1	hypothetical protein	-
- (54348)	54348..54399	-	52	NuclAT_2	-	-
- (54348)	54348..54399	-	52	NuclAT_2	-	-
- (54348)	54348..54399	-	52	NuclAT_2	-	-
- (54348)	54348..54399	-	52	NuclAT_2	-	-
LMH53_RS29105 (54885)	54885..55061	-	177	WP_001054900.1	hypothetical protein	-
LMH53_RS29110 (55270)	55270..55479	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
LMH53_RS29115 (55577)	55577..56191	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
LMH53_RS29120 (56267)	56267..58435	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99753	99753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T225753 WP_001303307.1 NZ_CP087632:53486-53638 [Klebsiella pneumoniae subsp. pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T225753 NZ_CP121298:c4484853-4484569 [Salmonella enterica subsp. enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT225753 NZ_CP087632:c53436-53374 [Klebsiella pneumoniae subsp. pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin