Detailed information of TA system TA225750

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 177716..177969
• Replicon: plasmid pDD02341-1
• Accession: NZ_CP087630
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LMH53_RS27810
• Protein ID: WP_001312851.1
• Coordinates: 177820..177969 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	177716..177775 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH53_RS27770 (173376)	173376..173441	-	66	Protein_226	helix-turn-helix domain-containing protein	-
LMH53_RS27775 (173494)	173494..174198	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH53_RS27780 (174223)	174223..174423	+	201	WP_072354025.1	hypothetical protein	-
LMH53_RS27785 (174443)	174443..175189	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LMH53_RS27790 (175244)	175244..175804	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LMH53_RS27795 (175936)	175936..176136	+	201	WP_015059022.1	hypothetical protein	-
LMH53_RS27800 (176522)	176522..177121	+	600	WP_032083981.1	PIN domain-containing protein	-
LMH53_RS27805 (177183)	177183..177515	+	333	WP_152916585.1	hypothetical protein	-
- (177716)	177716..177775	-	60	NuclAT_1	-	Antitoxin
- (177716)	177716..177775	-	60	NuclAT_1	-	Antitoxin
- (177716)	177716..177775	-	60	NuclAT_1	-	Antitoxin
- (177716)	177716..177775	-	60	NuclAT_1	-	Antitoxin
LMH53_RS27810 (177820)	177820..177969	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LMH53_RS27815 (178253)	178253..178501	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..178812	178812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225750 WP_001312851.1 NZ_CP087630:177820-177969 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225750 NZ_CP121298:2695578-2695862 [Salmonella enterica subsp. enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT225750 NZ_CP087630:c177775-177716 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin