Detailed information of TA system TA225747

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38344..38613
• Replicon: plasmid pDD02341-1
• Accession: NZ_CP087630
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LMH53_RS26890
• Protein ID: WP_001372321.1
• Coordinates: 38488..38613 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	38344..38409 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH53_RS26860	34054..34581	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LMH53_RS26865	34639..34872	+	234	WP_000006003.1	DUF905 family protein	-
LMH53_RS26870	34933..36956	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
LMH53_RS26875	37025..37459	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LMH53_RS26880	37456..38175	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	38187..38411	+	225	NuclAT_0	-	-
-	38187..38411	+	225	NuclAT_0	-	-
-	38187..38411	+	225	NuclAT_0	-	-
-	38187..38411	+	225	NuclAT_0	-	-
-	38344..38409	-	66	-	-	Antitoxin
LMH53_RS26885	38397..38546	+	150	Protein_50	plasmid maintenance protein Mok	-
LMH53_RS26890	38488..38613	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LMH53_RS26895	38932..39228	-	297	Protein_52	hypothetical protein	-
LMH53_RS26900	39528..39824	+	297	WP_001272251.1	hypothetical protein	-
LMH53_RS26905	39935..40756	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LMH53_RS26910	41053..41700	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LMH53_RS26915	41977..42360	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LMH53_RS26920	42551..43237	+	687	WP_015059009.1	PAS domain-containing protein	-
LMH53_RS26925	43331..43558	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..178812	178812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225747 WP_001372321.1 NZ_CP087630:38488-38613 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225747 NZ_CP121298:1195106-1195504 [Salmonella enterica subsp. enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT225747 NZ_CP087630:c38409-38344 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin