Detailed information of TA system TA022574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3908941..3909517
• Replicon: chromosome
• Accession: NC_010125
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A9H9Y8
• Locus tag: GDI_RS18070
• Protein ID: WP_012228624.1
• Coordinates: 3909236..3909517 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GDI_RS18065
• Protein ID: WP_012228623.1
• Coordinates: 3908941..3909219 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDI_RS18045	3904626..3905363	+	738	Protein_3612	hypothetical protein	-
GDI_RS18050	3905386..3906222	-	837	WP_012222260.1	IS5-like element ISGdi1 family transposase	-
GDI_RS18055	3906294..3907316	+	1023	Protein_3614	hypothetical protein	-
GDI_RS18060	3907528..3908883	+	1356	WP_012228622.1	inorganic phosphate transporter	-
GDI_RS18065	3908941..3909219	-	279	WP_012228623.1	HigA family addiction module antidote protein	Antitoxin
GDI_RS18070	3909236..3909517	-	282	WP_012228624.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GDI_RS18075	3909653..3910477	+	825	WP_012553437.1	IS5-like element ISGdi2 family transposase	-
GDI_RS18080	3910494..3911219	-	726	WP_012228625.1	amino acid ABC transporter ATP-binding protein	-
GDI_RS18085	3911216..3912769	-	1554	WP_012554504.1	ABC transporter substrate-binding protein/permease	-
GDI_RS18090	3912995..3913735	+	741	WP_012228627.1	class II aldolase/adducin family protein	-
GDI_RS18095	3913743..3914171	-	429	WP_012228628.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3905386..3910477	5091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10609.15 Da
• Isoelectric Point: 8.9890

>T22574 WP_012228624.1 NC_010125:c3909517-3909236 [Gluconacetobacter diazotrophicus PA1 5]
MIRTFADKRAEQVFTGKLPKGFQAQIANAARPKLRMLAAATVLDDLRVPPANRLEALAGDRQGQYSIRINDQFRICFVWR
DGDAYDVEIIDYH

Nucleotide

• Length: 282 bp

>T22574 NC_010125:c3909517-3909236 [Gluconacetobacter diazotrophicus PA1 5]
ATGATTCGAACCTTTGCCGACAAGCGAGCGGAACAGGTTTTTACCGGGAAGCTGCCAAAAGGCTTTCAGGCGCAGATCGC
CAATGCGGCACGACCGAAACTGCGCATGCTGGCAGCGGCGACCGTCCTCGACGATCTGCGGGTCCCGCCGGCAAACCGCC
TTGAGGCACTGGCTGGCGACCGGCAGGGGCAATACTCGATCCGCATCAACGACCAGTTCCGGATTTGCTTCGTATGGCGT
GACGGCGACGCCTATGATGTCGAAATCATCGATTATCACTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10217.63 Da
• Isoelectric Point: 5.7012

>AT22574 WP_012228623.1 NC_010125:c3909219-3908941 [Gluconacetobacter diazotrophicus PA1 5]
MTELHNVSAGDVLREEFMLPFGLSARALAAEIGVPANRLSDIVRGRRSVTADTAILLAERFGTSAEFWMNLQVAYDLEEA
REKLASRHRSTL

Nucleotide

• Length: 279 bp

>AT22574 NC_010125:c3909219-3908941 [Gluconacetobacter diazotrophicus PA1 5]
ATGACCGAACTTCACAACGTATCGGCGGGCGACGTGCTGCGGGAGGAATTCATGCTCCCGTTCGGCCTGTCCGCCCGCGC
CCTGGCGGCGGAAATCGGCGTGCCGGCCAACCGCCTGAGCGATATCGTGCGGGGGCGGCGGAGCGTGACGGCGGATACGG
CCATCCTGCTGGCGGAACGGTTTGGTACATCCGCCGAATTCTGGATGAACCTCCAGGTGGCCTACGACCTTGAAGAAGCA
CGGGAGAAGCTGGCAAGCCGGCACCGCTCCACCCTGTAA

Structures

Toxin

• AlphaFold DB: A9H9Y8