Detailed information of TA system TA225727

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 138761..139014
• Replicon: plasmid pDD02280-2
• Accession: NZ_CP087625
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LMH50_RS29040
• Protein ID: WP_001312851.1
• Coordinates: 138865..139014 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	138761..138820 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH50_RS28995 (133794)	133794..134099	+	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-
LMH50_RS29000 (134119)	134119..134484	+	366	Protein_168	type IV conjugative transfer system protein TraE	-
LMH50_RS29005 (134539)	134539..135243	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMH50_RS29010 (135268)	135268..135468	+	201	WP_072354025.1	hypothetical protein	-
LMH50_RS29015 (135488)	135488..136234	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LMH50_RS29020 (136289)	136289..136849	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LMH50_RS29025 (136981)	136981..137181	+	201	WP_015059022.1	hypothetical protein	-
LMH50_RS29030 (137567)	137567..138166	+	600	WP_032083981.1	PIN domain-containing protein	-
LMH50_RS29035 (138228)	138228..138560	+	333	WP_152916585.1	hypothetical protein	-
- (138761)	138761..138820	-	60	NuclAT_1	-	Antitoxin
- (138761)	138761..138820	-	60	NuclAT_1	-	Antitoxin
- (138761)	138761..138820	-	60	NuclAT_1	-	Antitoxin
- (138761)	138761..138820	-	60	NuclAT_1	-	Antitoxin
LMH50_RS29040 (138865)	138865..139014	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LMH50_RS29045 (139298)	139298..139546	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..139857	139857
	-	flank	IS/Tn	-	-	134539..135243	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225727 WP_001312851.1 NZ_CP087625:138865-139014 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225727 NZ_CP121296:1360942-1361253 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT225727 NZ_CP087625:c138820-138761 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin