Detailed information of TA system TA225724

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36249..36518
• Replicon: plasmid pDD02280-2
• Accession: NZ_CP087625
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LMH50_RS28400
• Protein ID: WP_001372321.1
• Coordinates: 36393..36518 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36249..36314 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH50_RS28370	31959..32486	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LMH50_RS28375	32544..32777	+	234	WP_000006003.1	DUF905 family protein	-
LMH50_RS28380	32838..34861	+	2024	Protein_44	ParB/RepB/Spo0J family partition protein	-
LMH50_RS28385	34930..35364	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LMH50_RS28390	35361..36080	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36092..36316	+	225	NuclAT_0	-	-
-	36092..36316	+	225	NuclAT_0	-	-
-	36092..36316	+	225	NuclAT_0	-	-
-	36092..36316	+	225	NuclAT_0	-	-
-	36249..36314	-	66	-	-	Antitoxin
LMH50_RS28395	36302..36451	+	150	Protein_47	plasmid maintenance protein Mok	-
LMH50_RS28400	36393..36518	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LMH50_RS28405	36837..37133	-	297	Protein_49	hypothetical protein	-
LMH50_RS28410	37433..37729	+	297	WP_001272251.1	hypothetical protein	-
LMH50_RS28415	37840..38661	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LMH50_RS28420	38958..39605	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LMH50_RS28425	39882..40265	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LMH50_RS28430	40546..41250	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..139857	139857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225724 WP_001372321.1 NZ_CP087625:36393-36518 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225724 NZ_CP121296:963448-963864 [Salmonella enterica subsp. enterica]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT225724 NZ_CP087625:c36314-36249 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin