Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3854101..3854698 Replicon chromosome
Accession NZ_CP087618
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LMH58_RS18665 Protein ID WP_004142563.1
Coordinates 3854381..3854698 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LMH58_RS18660 Protein ID WP_004142561.1
Coordinates 3854101..3854388 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH58_RS18630 (3850181) 3850181..3850429 + 249 WP_020325243.1 DUF1158 domain-containing protein -
LMH58_RS18635 (3850447) 3850447..3850788 - 342 WP_117074703.1 RamA family antibiotic efflux transcriptional regulator -
LMH58_RS18640 (3850819) 3850819..3851934 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
LMH58_RS18645 (3852114) 3852114..3852695 + 582 WP_247440304.1 TetR/AcrR family transcriptional regulator -
LMH58_RS18650 (3852695) 3852695..3853063 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
LMH58_RS18655 (3853183) 3853183..3853836 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LMH58_RS18660 (3854101) 3854101..3854388 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LMH58_RS18665 (3854381) 3854381..3854698 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMH58_RS18670 (3854883) 3854883..3855926 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
LMH58_RS18675 (3856596) 3856596..3857462 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LMH58_RS18680 (3857571) 3857571..3858998 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T225692 WP_004142563.1 NZ_CP087618:c3854698-3854381 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T225692 NZ_CP121294:c476204-475953 [Escherichia coli O155:H21]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT225692 WP_004142561.1 NZ_CP087618:c3854388-3854101 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT225692 NZ_CP121294:c475956-475702 [Escherichia coli O155:H21]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References