Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 42189..42913 | Replicon | plasmid pDD02172-3 |
| Accession | NZ_CP087614 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD02172 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A2J4YLV3 |
| Locus tag | LMH59_RS28000 | Protein ID | WP_023292165.1 |
| Coordinates | 42189..42500 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | A0A7G3NP42 |
| Locus tag | LMH59_RS28005 | Protein ID | WP_023317816.1 |
| Coordinates | 42497..42913 (+) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH59_RS27955 (LMH59_27955) | 37402..37833 | + | 432 | WP_004152657.1 | conjugation system SOS inhibitor PsiB | - |
| LMH59_RS27960 (LMH59_27960) | 37830..38552 | + | 723 | WP_022631512.1 | plasmid SOS inhibition protein A | - |
| LMH59_RS27965 (LMH59_27965) | 38556..38879 | + | 324 | WP_004199367.1 | hypothetical protein | - |
| LMH59_RS27970 (LMH59_27970) | 38990..39364 | + | 375 | WP_050584024.1 | hypothetical protein | - |
| LMH59_RS27975 (LMH59_27975) | 39440..39736 | + | 297 | WP_023317812.1 | hypothetical protein | - |
| LMH59_RS27980 (LMH59_27980) | 40392..40664 | + | 273 | WP_023317813.1 | hypothetical protein | - |
| LMH59_RS27985 (LMH59_27985) | 40661..41011 | + | 351 | WP_104513727.1 | hypothetical protein | - |
| LMH59_RS27990 (LMH59_27990) | 41641..41847 | + | 207 | WP_023317814.1 | hypothetical protein | - |
| LMH59_RS27995 (LMH59_27995) | 41858..42082 | + | 225 | WP_023317815.1 | hypothetical protein | - |
| LMH59_RS28000 (LMH59_28000) | 42189..42500 | + | 312 | WP_023292165.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LMH59_RS28005 (LMH59_28005) | 42497..42913 | + | 417 | WP_023317816.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LMH59_RS28010 (LMH59_28010) | 43748..44101 | + | 354 | WP_023317817.1 | hypothetical protein | - |
| LMH59_RS28015 (LMH59_28015) | 44158..44505 | + | 348 | WP_023317818.1 | hypothetical protein | - |
| LMH59_RS28020 (LMH59_28020) | 44600..44746 | + | 147 | WP_004152750.1 | hypothetical protein | - |
| LMH59_RS28025 (LMH59_28025) | 44796..45629 | + | 834 | WP_023317819.1 | N-6 DNA methylase | - |
| LMH59_RS28030 (LMH59_28030) | 46447..47268 | + | 822 | WP_023317820.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..81442 | 81442 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12497.32 Da Isoelectric Point: 9.9242
>T225679 WP_023292165.1 NZ_CP087614:42189-42500 [Klebsiella pneumoniae subsp. pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE
Download Length: 312 bp
>T225679 NZ_CP121269:3553468-3553767 [Enterobacter hormaechei subsp. xiangfangensis]
ATGATTAATCATTTTTGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA
ATGATTAATCATTTTTGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA
Antitoxin
Download Length: 139 a.a. Molecular weight: 15458.60 Da Isoelectric Point: 4.4803
>AT225679 WP_023317816.1 NZ_CP087614:42497-42913 [Klebsiella pneumoniae subsp. pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHEPDSPLVDMLTARIDAWEDTAVEFEEFNTRIEAGKNGV
SLLRVLMQQRGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHEPDSPLVDMLTARIDAWEDTAVEFEEFNTRIEAGKNGV
SLLRVLMQQRGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD
Download Length: 417 bp
>AT225679 NZ_CP121269:3553778-3554143 [Enterobacter hormaechei subsp. xiangfangensis]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAACACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAACACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4YLV3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3NP42 |