Detailed information of TA system TA225664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3885094..3885691
• Replicon: chromosome
• Accession: NZ_CP087611
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: LMH59_RS18790
• Protein ID: WP_004142563.1
• Coordinates: 3885374..3885691 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LMH59_RS18785
• Protein ID: WP_004142561.1
• Coordinates: 3885094..3885381 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH59_RS18755 (3881174)	3881174..3881422	+	249	WP_020325243.1	DUF1158 domain-containing protein	-
LMH59_RS18760 (3881440)	3881440..3881781	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LMH59_RS18765 (3881812)	3881812..3882927	-	1116	WP_020325248.1	MBL fold metallo-hydrolase	-
LMH59_RS18770 (3883107)	3883107..3883688	+	582	WP_247333476.1	TetR/AcrR family transcriptional regulator	-
LMH59_RS18775 (3883688)	3883688..3884056	+	369	WP_020325252.1	MmcQ/YjbR family DNA-binding protein	-
LMH59_RS18780 (3884176)	3884176..3884829	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LMH59_RS18785 (3885094)	3885094..3885381	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LMH59_RS18790 (3885374)	3885374..3885691	-	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH59_RS18795 (3885876)	3885876..3886919	-	1044	WP_020325241.1	DUF2157 domain-containing protein	-
LMH59_RS18800 (3887588)	3887588..3888454	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LMH59_RS18805 (3888563)	3888563..3889990	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T225664 WP_004142563.1 NZ_CP087611:c3885691-3885374 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T225664 NZ_CP121262:c4070955-4070668 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT225664 WP_004142561.1 NZ_CP087611:c3885381-3885094 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT225664 NZ_CP121262:c4070681-4070379 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8

Antitoxin

• AlphaFold DB: A0A0S3DIQ1