Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 49658..50385 | Replicon | plasmid pDD01304-1 |
| Accession | NZ_CP087607 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD01304 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A5Q9LNL2 |
| Locus tag | LMH54_RS27285 | Protein ID | WP_004118600.1 |
| Coordinates | 49658..49969 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | LMH54_RS27290 | Protein ID | WP_000990392.1 |
| Coordinates | 49966..50385 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH54_RS27265 (LMH54_27265) | 46172..46891 | + | 720 | WP_001102107.1 | (Fe-S)-binding protein | - |
| LMH54_RS27270 (LMH54_27270) | 46902..48329 | + | 1428 | WP_000023895.1 | LutB/LldF family L-lactate oxidation iron-sulfur protein | - |
| LMH54_RS27275 (LMH54_27275) | 48322..49017 | + | 696 | WP_004118595.1 | lactate utilization protein C | - |
| LMH54_RS27280 (LMH54_27280) | 49067..49447 | - | 381 | Protein_46 | gluconate permease | - |
| LMH54_RS27285 (LMH54_27285) | 49658..49969 | + | 312 | WP_004118600.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LMH54_RS27290 (LMH54_27290) | 49966..50385 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LMH54_RS27295 (LMH54_27295) | 50422..51624 | - | 1203 | WP_003031541.1 | hypothetical protein | - |
| LMH54_RS27300 (LMH54_27300) | 51617..51946 | - | 330 | WP_004118613.1 | hypothetical protein | - |
| LMH54_RS27305 (LMH54_27305) | 51943..52602 | - | 660 | WP_000078540.1 | hypothetical protein | - |
| LMH54_RS27310 (LMH54_27310) | 53026..53382 | - | 357 | WP_077253206.1 | Ref family recombination enhancement nuclease | - |
| LMH54_RS27315 (LMH54_27315) | 53502..53642 | - | 141 | WP_004118614.1 | hypothetical protein | - |
| LMH54_RS27320 (LMH54_27320) | 53935..54288 | + | 354 | WP_001114073.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| LMH54_RS27325 (LMH54_27325) | 54336..54698 | + | 363 | WP_020324593.1 | arsenite efflux transporter metallochaperone ArsD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / catA2 / tet(A) / dfrA1 / qacE / sul1 / qnrS1 / blaLAP-2 | - | 1..210574 | 210574 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12386.14 Da Isoelectric Point: 10.0935
>T225645 WP_004118600.1 NZ_CP087607:49658-49969 [Klebsiella pneumoniae subsp. pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T225645 NZ_CP121247:c52867-52508 [Arcanobacterium wilhelmae]
ATGTGGGAGATTATCCTGATGAAACAAGTTGAGGACTGGCTCCTCAACCTCACCGACGACGATTATGATCTCGTTGCCGC
CGCGATCACGCGCCTCGAAACCGCCGGGCCAGCGCTTGGTCGTCCCACTGCAGACCACATCAAAGCATCACGTCATCACA
ACATGAAAGAGCTAAGGCCGGGTTCTCGTGGACGAAGCAAGATCCGCATCCTCTTCGCCTTCGACCCTCACCGCCAAGCA
GTCCTCCTCATTGCGGGAGATAAAGCCGACAAATGGAGCGAGTGGTACAAAACCAACATTCCTCGAGCTGACGCACTGTT
CGACGAATGGCTCCTCCAACTCAAAACCTACGAAGAATAG
ATGTGGGAGATTATCCTGATGAAACAAGTTGAGGACTGGCTCCTCAACCTCACCGACGACGATTATGATCTCGTTGCCGC
CGCGATCACGCGCCTCGAAACCGCCGGGCCAGCGCTTGGTCGTCCCACTGCAGACCACATCAAAGCATCACGTCATCACA
ACATGAAAGAGCTAAGGCCGGGTTCTCGTGGACGAAGCAAGATCCGCATCCTCTTCGCCTTCGACCCTCACCGCCAAGCA
GTCCTCCTCATTGCGGGAGATAAAGCCGACAAATGGAGCGAGTGGTACAAAACCAACATTCCTCGAGCTGACGCACTGTT
CGACGAATGGCTCCTCCAACTCAAAACCTACGAAGAATAG
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT225645 WP_000990392.1 NZ_CP087607:49966-50385 [Klebsiella pneumoniae subsp. pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT225645 NZ_CP121247:c52494-52186 [Arcanobacterium wilhelmae]
ATGGAAACGACATCCTGGAAGGACATGAAGGCCCAGCGTCAGCCACGCCCAGAACGAGTCGCTGAACTCGTACAGCAGAT
GGAGGCGGAAGAGCGCGCATATTCGCTTCGCCAAATCCGCGAATCACTTGGTGTGACACAATCCGAAGTTGCGCAGCGTA
TGAACCTTACCCAGCCCACGATCTCCGCGCTTGAAAAAGGTGAACTCGGGCGCTCAGGGCTCGATACCCTGCGCGCTTAC
GTCACCGCTCTCGGTGGAGCTGTCGAAGTCGTCGCGACGTTTGGTAACCAGAAGCTCATCCTCGGCTGA
ATGGAAACGACATCCTGGAAGGACATGAAGGCCCAGCGTCAGCCACGCCCAGAACGAGTCGCTGAACTCGTACAGCAGAT
GGAGGCGGAAGAGCGCGCATATTCGCTTCGCCAAATCCGCGAATCACTTGGTGTGACACAATCCGAAGTTGCGCAGCGTA
TGAACCTTACCCAGCCCACGATCTCCGCGCTTGAAAAAGGTGAACTCGGGCGCTCAGGGCTCGATACCCTGCGCGCTTAC
GTCACCGCTCTCGGTGGAGCTGTCGAAGTCGTCGCGACGTTTGGTAACCAGAAGCTCATCCTCGGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Q9LNL2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |