Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3788025..3788622 Replicon chromosome
Accession NZ_CP087580
Organism Klebsiella quasipneumoniae strain OSUCMP263AKPC

Toxin (Protein)


Gene name higB Uniprot ID A0A3T0QUS6
Locus tag LO541_RS18480 Protein ID WP_017899012.1
Coordinates 3788305..3788622 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3T0QUV3
Locus tag LO541_RS18475 Protein ID WP_017899013.1
Coordinates 3788025..3788312 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO541_RS18445 (3784104) 3784104..3784352 + 249 WP_130938745.1 DUF1158 domain-containing protein -
LO541_RS18450 (3784370) 3784370..3784711 - 342 WP_004202492.1 RamA family antibiotic efflux transcriptional regulator -
LO541_RS18455 (3784742) 3784742..3785857 - 1116 WP_165453183.1 MBL fold metallo-hydrolase -
LO541_RS18460 (3786037) 3786037..3786626 + 590 Protein_3620 TetR/AcrR family transcriptional regulator -
LO541_RS18465 (3786626) 3786626..3786994 + 369 WP_004202487.1 MmcQ/YjbR family DNA-binding protein -
LO541_RS18470 (3787114) 3787114..3787767 + 654 WP_004202485.1 oxygen-insensitive NAD(P)H nitroreductase -
LO541_RS18475 (3788025) 3788025..3788312 - 288 WP_017899013.1 helix-turn-helix transcriptional regulator Antitoxin
LO541_RS18480 (3788305) 3788305..3788622 - 318 WP_017899012.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LO541_RS18485 (3788807) 3788807..3789850 - 1044 WP_060656299.1 hypothetical protein -
LO541_RS18490 (3790388) 3790388..3791254 - 867 WP_130938743.1 helix-turn-helix transcriptional regulator -
LO541_RS18495 (3791363) 3791363..3792790 + 1428 WP_053810681.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12185.52 Da        Isoelectric Point: 11.2767

>T225590 WP_017899012.1 NZ_CP087580:c3788622-3788305 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKTPLSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T225590 NZ_CP121199:c4180375-4180088 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10840.52 Da        Isoelectric Point: 10.2213

>AT225590 WP_017899013.1 NZ_CP087580:c3788312-3788025 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT225590 NZ_CP121199:c4180101-4179799 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0QUS6

Antitoxin

Source ID Structure
AlphaFold DB A0A3T0QUV3

References