Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3721353..3722080 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag KUA31_RS18330 Protein ID WP_000547563.1
Coordinates 3721769..3722080 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KUA31_RS18325 Protein ID WP_000126294.1
Coordinates 3721353..3721772 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS18305 (3716403) 3716403..3716930 - 528 WP_001078777.1 electron transport protein HydN -
KUA31_RS18310 (3717079) 3717079..3718089 - 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
KUA31_RS18315 (3718349) 3718349..3719806 + 1458 WP_075826801.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
KUA31_RS18320 (3719815) 3719815..3721239 + 1425 WP_000110365.1 6-phospho-beta-glucosidase AscB -
KUA31_RS18325 (3721353) 3721353..3721772 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
KUA31_RS18330 (3721769) 3721769..3722080 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
KUA31_RS18335 (3722253) 3722253..3722996 - 744 WP_058101263.1 hypothetical protein -
KUA31_RS18340 (3723039) 3723039..3723509 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
KUA31_RS18345 (3723502) 3723502..3723912 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
KUA31_RS18350 (3723909) 3723909..3724676 - 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
KUA31_RS18355 (3724676) 3724676..3725218 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
KUA31_RS18360 (3725228) 3725228..3726937 - 1710 WP_063625439.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T225573 WP_000547563.1 NZ_CP087578:c3722080-3721769 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T225573 NZ_CP121199:241159-241266 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT225573 WP_000126294.1 NZ_CP087578:c3721772-3721353 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT225573 NZ_CP121199:c241110-241047 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATAACTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References