Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2587080..2587603 Replicon chromosome
Accession NC_010079
Organism Staphylococcus aureus subsp. aureus USA300 TCH1516

Toxin (Protein)


Gene name yoeB Uniprot ID X5DYX7
Locus tag USA300HOU_RS13320 Protein ID WP_000074048.1
Coordinates 2587080..2587346 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag USA300HOU_RS13325 Protein ID WP_000587616.1
Coordinates 2587346..2587603 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USA300HOU_RS13300 2582387..2583574 - 1188 WP_000026194.1 MFS transporter -
USA300HOU_RS13305 2583970..2584746 - 777 WP_000072147.1 iron export ABC transporter permease subunit FetB -
USA300HOU_RS13310 2584739..2585401 - 663 WP_000923520.1 ATP-binding cassette domain-containing protein -
USA300HOU_RS13315 2585649..2586725 - 1077 WP_001076671.1 M42 family metallopeptidase -
USA300HOU_RS13320 2587080..2587346 - 267 WP_000074048.1 Txe/YoeB family addiction module toxin Toxin
USA300HOU_RS13325 2587346..2587603 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
USA300HOU_RS13330 2587709..2587903 - 195 WP_001790145.1 hypothetical protein -
USA300HOU_RS13335 2587962..2588417 + 456 WP_000732082.1 DUF1307 domain-containing protein -
USA300HOU_RS13340 2588585..2590162 - 1578 WP_000143503.1 FMN-binding glutamate synthase family protein -
USA300HOU_RS13345 2590357..2591106 + 750 WP_000072444.1 hypothetical protein -
USA300HOU_RS13350 2591330..2592523 - 1194 WP_000675401.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10648.23 Da        Isoelectric Point: 10.1384

>T22556 WP_000074048.1 NC_010079:c2587346-2587080 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T22556 NC_010079:c2587346-2587080 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT22556 WP_000587616.1 NC_010079:c2587603-2587346 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT22556 NC_010079:c2587603-2587346 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7V6W
PDB 7V5Y
PDB 7V5Z

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References