Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1050585..1051126 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KUA31_RS05345 Protein ID WP_000615976.1
Coordinates 1050585..1050863 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KUA31_RS05350 Protein ID WP_000729704.1
Coordinates 1050866..1051126 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS05330 (1048167) 1048167..1048745 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KUA31_RS05335 (1048951) 1048951..1049718 + 768 WP_000333380.1 class II glutamine amidotransferase -
KUA31_RS05340 (1049689) 1049689..1050429 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KUA31_RS05345 (1050585) 1050585..1050863 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KUA31_RS05350 (1050866) 1050866..1051126 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KUA31_RS05355 (1051312) 1051312..1052085 + 774 WP_000543897.1 C40 family peptidase -
KUA31_RS05360 (1052142) 1052142..1052498 - 357 WP_125291427.1 type II toxin-antitoxin system HicB family antitoxin -
KUA31_RS05365 (1052491) 1052491..1052769 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KUA31_RS05370 (1052874) 1052874..1054586 - 1713 Protein_955 flagellar biosynthesis protein FlhA -
KUA31_RS05375 (1054558) 1054558..1055343 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T225549 WP_000615976.1 NZ_CP087578:c1050863-1050585 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T225549 NZ_CP121160:668670-669515 [Streptococcus agalactiae]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTCATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TTTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATTACAGCTAT
TAAAGAAAGTGATGACTACGGTGGCTATCGAGCAACCATTTTATGCCAACTTGAGAATATTAAACAAATTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCACAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGAGAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTTCAAACCATCTATTCACGCAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAATTGAAAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGAAAAGATTATTGAACTACTCGAAAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAGGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTCTCATAACAGTTCTCATTTCTATAAATTCTGATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT225549 WP_000729704.1 NZ_CP087578:c1051126-1050866 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT225549 NZ_CP121160:668083-668673 [Streptococcus agalactiae]
ATGTCTAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGTATTATCACCTATAAAGATTGTAAAGCTCTAAA
GATTCCAACAATATATTTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAATATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References