Detailed information of TA system TA225542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25114..25378
• Replicon: plasmid pCMP42-2
• Accession: NZ_CP087576
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: KUA31_RS00260
• Protein ID: WP_001387489.1
• Coordinates: 25226..25378 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	25114..25174 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS00230 (21224)	21224..21382	+	159	Protein_32	ash family protein	-
KUA31_RS00235 (21420)	21420..21707	+	288	WP_000074855.1	conjugal transfer protein TraA	-
KUA31_RS00240 (22149)	22149..22457	+	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
KUA31_RS00250 (23221)	23221..24378	-	1158	Protein_36	IS1380-like element ISEcp1 family transposase	-
KUA31_RS00255 (24531)	24531..24905	-	375	WP_223349261.1	hypothetical protein	-
- (25114)	25114..25174	-	61	NuclAT_0	-	Antitoxin
- (25114)	25114..25174	-	61	NuclAT_0	-	Antitoxin
- (25114)	25114..25174	-	61	NuclAT_0	-	Antitoxin
- (25114)	25114..25174	-	61	NuclAT_0	-	Antitoxin
KUA31_RS00260 (25226)	25226..25378	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
KUA31_RS00265 (25450)	25450..25701	-	252	WP_001291964.1	hypothetical protein	-
KUA31_RS00270 (26001)	26001..26297	+	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
KUA31_RS00275 (26362)	26362..26538	-	177	WP_001054897.1	hypothetical protein	-
KUA31_RS00280 (26930)	26930..27139	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KUA31_RS00285 (27211)	27211..27309	-	99	Protein_43	ethanolamine utilization protein EutE	-
KUA31_RS00290 (27364)	27364..28061	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KUA31_RS00295 (28077)	28077..28190	+	114	Protein_45	hypothetical protein	-
KUA31_RS00300 (28514)	28514..29659	+	1146	WP_065644637.1	class C beta-lactamase CMY-141	-
KUA31_RS00305 (29753)	29753..30286	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-141	-	1..51705	51705
	-	inside	IScluster/Tn	blaCMY-141	-	22890..29659	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T225542 WP_001387489.1 NZ_CP087576:25226-25378 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T225542 NZ_CP121158:c52397-52092 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT225542 NZ_CP087576:c25174-25114 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin