Detailed information of TA system TA225538

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108394..108663
• Replicon: plasmid p1OXA77
• Accession: NZ_CP087571
• Organism: Escherichia coli strain 114

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LOS76_RS23665
• Protein ID: WP_001372321.1
• Coordinates: 108538..108663 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	108394..108459 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOS76_RS23630	104161..104700	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
LOS76_RS23635	104763..104996	+	234	WP_000005990.1	DUF905 family protein	-
LOS76_RS23640	105062..107020	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
LOS76_RS23645	107075..107509	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
LOS76_RS23650	107506..108268	+	763	Protein_130	plasmid SOS inhibition protein A	-
LOS76_RS23655	108237..108425	-	189	WP_001299721.1	hypothetical protein	-
-	108237..108461	+	225	NuclAT_0	-	-
-	108237..108461	+	225	NuclAT_0	-	-
-	108237..108461	+	225	NuclAT_0	-	-
-	108237..108461	+	225	NuclAT_0	-	-
-	108394..108459	-	66	-	-	Antitoxin
LOS76_RS23660	108447..108596	+	150	Protein_132	plasmid maintenance protein Mok	-
LOS76_RS23665	108538..108663	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LOS76_RS23670	108883..109113	+	231	WP_071587244.1	hypothetical protein	-
LOS76_RS23675	109111..109284	-	174	Protein_135	hypothetical protein	-
LOS76_RS23680	109354..109560	+	207	WP_000547939.1	hypothetical protein	-
LOS76_RS23685	109585..109872	+	288	WP_000107535.1	hypothetical protein	-
LOS76_RS23690	109994..110815	+	822	WP_001234469.1	DUF932 domain-containing protein	-
LOS76_RS23695	111112..111714	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
LOS76_RS23700	112035..112418	+	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LOS76_RS23705	112605..113294	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / mph(A) / aac(3)-IId / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..151915	151915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225538 WP_001372321.1 NZ_CP087571:108538-108663 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225538 NZ_CP121157:3720959-3721237 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT225538 NZ_CP087571:c108459-108394 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin