Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2530232..2530749 Replicon chromosome
Accession NC_010079
Organism Staphylococcus aureus subsp. aureus USA300 TCH1516

Toxin (Protein)


Gene name yoeB Uniprot ID T1YC79
Locus tag USA300HOU_RS13010 Protein ID WP_000113262.1
Coordinates 2530232..2530498 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A5A8VWS3
Locus tag USA300HOU_RS13015 Protein ID WP_000584502.1
Coordinates 2530498..2530749 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USA300HOU_RS12985 2525658..2526194 - 537 WP_000169317.1 GNAT family N-acetyltransferase -
USA300HOU_RS12990 2526427..2527251 - 825 WP_000572040.1 formate/nitrite transporter family protein -
USA300HOU_RS12995 2527468..2527650 - 183 WP_000230294.1 hypothetical protein -
USA300HOU_RS13000 2527734..2528201 - 468 WP_000153344.1 SRPBCC domain-containing protein -
USA300HOU_RS13005 2528387..2529937 - 1551 WP_000727762.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
USA300HOU_RS15830 2530126..2530197 - 72 Protein_2470 hypothetical protein -
USA300HOU_RS13010 2530232..2530498 - 267 WP_000113262.1 Txe/YoeB family addiction module toxin Toxin
USA300HOU_RS13015 2530498..2530749 - 252 WP_000584502.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
USA300HOU_RS13020 2530914..2531015 - 102 WP_001791744.1 hypothetical protein -
USA300HOU_RS13025 2531022..2531621 - 600 WP_000162813.1 DsbA family protein -
USA300HOU_RS13030 2531640..2531999 - 360 WP_000819847.1 DUF4467 domain-containing protein -
USA300HOU_RS13035 2532254..2533504 + 1251 WP_001012222.1 FemA/FemB family glycyltransferase FmhA -
USA300HOU_RS13040 2533601..2534332 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
USA300HOU_RS13045 2534329..2535048 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10437.95 Da        Isoelectric Point: 9.9143

>T22553 WP_000113262.1 NC_010079:c2530498-2530232 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T22553 NC_010079:c2530498-2530232 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9417.65 Da        Isoelectric Point: 4.4702

>AT22553 WP_000584502.1 NC_010079:c2530749-2530498 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT22553 NC_010079:c2530749-2530498 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7CUA
PDB 6L8E

Antitoxin

Source ID Structure
AlphaFold DB A0A5A8VWS3

References