Detailed information of TA system TA225518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2488096..2488823
• Replicon: chromosome
• Accession: NZ_CP087570
• Organism: Escherichia coli strain 114

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LOS76_RS11955
• Protein ID: WP_000547564.1
• Coordinates: 2488096..2488407 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOS76_RS11960
• Protein ID: WP_000126294.1
• Coordinates: 2488404..2488823 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOS76_RS11925 (2483238)	2483238..2484947	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LOS76_RS11930 (2484957)	2484957..2485499	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LOS76_RS11935 (2485499)	2485499..2486266	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LOS76_RS11940 (2486263)	2486263..2486673	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LOS76_RS11945 (2486666)	2486666..2487136	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
LOS76_RS11950 (2487161)	2487161..2487922	+	762	WP_001026446.1	hypothetical protein	-
LOS76_RS11955 (2488096)	2488096..2488407	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LOS76_RS11960 (2488404)	2488404..2488823	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LOS76_RS11965 (2488937)	2488937..2490361	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LOS76_RS11970 (2490370)	2490370..2491827	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LOS76_RS11975 (2492087)	2492087..2493097	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LOS76_RS11980 (2493246)	2493246..2493773	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T225518 WP_000547564.1 NZ_CP087570:2488096-2488407 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T225518 NZ_CP121155:3644635-3644853 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT225518 WP_000126294.1 NZ_CP087570:2488404-2488823 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT225518 NZ_CP121155:3644235-3644609 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5