Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2032599..2033398 Replicon chromosome
Accession NZ_CP087570
Organism Escherichia coli strain 114

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LOS76_RS09745 Protein ID WP_000347251.1
Coordinates 2032599..2033063 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LOS76_RS09750 Protein ID WP_001307405.1
Coordinates 2033063..2033398 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOS76_RS09715 (2027600) 2027600..2028034 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LOS76_RS09720 (2028052) 2028052..2028930 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LOS76_RS09725 (2028920) 2028920..2029699 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LOS76_RS09730 (2029710) 2029710..2030183 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LOS76_RS09735 (2030206) 2030206..2031486 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
LOS76_RS09740 (2031735) 2031735..2032544 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LOS76_RS09745 (2032599) 2032599..2033063 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LOS76_RS09750 (2033063) 2033063..2033398 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LOS76_RS09755 (2033547) 2033547..2035118 - 1572 WP_001273761.1 galactarate dehydratase -
LOS76_RS09760 (2035493) 2035493..2036827 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
LOS76_RS09765 (2036843) 2036843..2037613 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T225514 WP_000347251.1 NZ_CP087570:c2033063-2032599 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T225514 NZ_CP121155:c2394292-2394014 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT225514 WP_001307405.1 NZ_CP087570:c2033398-2033063 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT225514 NZ_CP121155:c2394014-2393730 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References