Detailed information of TA system TA225497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4286262..4286956
• Replicon: chromosome
• Accession: NZ_CP087564
• Organism: Escherichia coli strain CM2010-0578/1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LOF66_RS21405
• Protein ID: WP_001263489.1
• Coordinates: 4286558..4286956 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LOF66_RS21400
• Protein ID: WP_000554758.1
• Coordinates: 4286262..4286555 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOF66_RS21375 (4282079)	4282079..4282546	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
LOF66_RS21380 (4282566)	4282566..4283282	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
LOF66_RS21385 (4283295)	4283295..4284158	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
LOF66_RS21390 (4284161)	4284161..4285084	+	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
LOF66_RS21395 (4285155)	4285155..4286210	+	1056	WP_001226164.1	DNA polymerase IV	-
LOF66_RS21400 (4286262)	4286262..4286555	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LOF66_RS21405 (4286558)	4286558..4286956	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LOF66_RS21410 (4286966)	4286966..4287418	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LOF66_RS21415 (4287736)	4287736..4287942	+	207	Protein_4180	RtcB family protein	-
LOF66_RS21420 (4287938)	4287938..4288459	+	522	Protein_4181	peptide chain release factor H	-
LOF66_RS21425 (4288516)	4288516..4289973	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LOF66_RS21430 (4290234)	4290234..4290692	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4291288)	4291288..4291368	+	81	NuclAT_13	-	-
- (4291288)	4291288..4291368	+	81	NuclAT_13	-	-
- (4291288)	4291288..4291368	+	81	NuclAT_13	-	-
- (4291288)	4291288..4291368	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T225497 WP_001263489.1 NZ_CP087564:4286558-4286956 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T225497 NZ_CP121153:c4305863-4305513 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT225497 WP_000554758.1 NZ_CP087564:4286262-4286555 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT225497 NZ_CP121153:c4306108-4305857 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3