Detailed information of TA system TA225489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2441282..2441975
• Replicon: chromosome
• Accession: NZ_CP087564
• Organism: Escherichia coli strain CM2010-0578/1

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LOF66_RS12395
• Protein ID: WP_000415584.1
• Coordinates: 2441679..2441975 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: LOF66_RS12390
• Protein ID: WP_274480475.1
• Coordinates: 2441282..2441677 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOF66_RS12380 (2437146)	2437146..2439404	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
LOF66_RS12385 (2439542)	2439542..2441149	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LOF66_RS12390 (2441282)	2441282..2441677	-	396	WP_274480475.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LOF66_RS12395 (2441679)	2441679..2441975	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LOF66_RS12400 (2442180)	2442180..2442662	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
LOF66_RS12405 (2442715)	2442715..2443107	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LOF66_RS12410 (2443259)	2443259..2443918	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
LOF66_RS12415 (2443915)	2443915..2445264	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LOF66_RS12420 (2445310)	2445310..2445642	-	333	WP_000917684.1	DUF2645 family protein	-
LOF66_RS12425 (2445961)	2445961..2446542	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LOF66_RS12430 (2446573)	2446573..2446887	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T225489 WP_000415584.1 NZ_CP087564:c2441975-2441679 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T225489 NZ_CP121153:c2411132-2410854 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14702.16 Da
• Isoelectric Point: 9.7805

>AT225489 WP_274480475.1 NZ_CP087564:c2441677-2441282 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAKTVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT225489 NZ_CP121153:c2410854-2410570 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• PDB: 3HI2