Detailed information of TA system TA225469

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 509468..509839
• Replicon: chromosome
• Accession: NZ_CP087564
• Organism: Escherichia coli strain CM2010-0578/1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: LOF66_RS02650
• Protein ID: WP_001443846.1
• Coordinates: 509690..509839 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	509468..509646 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOF66_RS02620 (505220)	505220..505393	+	174	WP_001296046.1	protein YnaL	-
LOF66_RS02625 (505423)	505423..506796	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
LOF66_RS02630 (506925)	506925..507860	-	936	WP_001614527.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LOF66_RS02635 (507912)	507912..509147	-	1236	WP_000040839.1	site-specific integrase	-
LOF66_RS02640 (509149)	509149..509364	-	216	WP_000079604.1	excisionase XisR	-
- (509468)	509468..509646	+	179	NuclAT_0	-	Antitoxin
- (509468)	509468..509646	+	179	NuclAT_0	-	Antitoxin
- (509468)	509468..509646	+	179	NuclAT_0	-	Antitoxin
- (509468)	509468..509646	+	179	NuclAT_0	-	Antitoxin
LOF66_RS02645 (509464)	509464..509652	-	189	WP_001302840.1	DUF1187 family protein	-
LOF66_RS02650 (509690)	509690..509839	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LOF66_RS02655 (509895)	509895..510704	-	810	WP_000166313.1	recombination protein RecT	-
LOF66_RS02660 (510697)	510697..513297	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
LOF66_RS02665 (513399)	513399..513674	-	276	WP_000632297.1	protein RacC	-
LOF66_RS02670 (513749)	513749..513919	-	171	WP_001352098.1	YdaE family protein	-
LOF66_RS02675 (513919)	513919..514140	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	507912..532794	24882

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T225469 WP_001443846.1 NZ_CP087564:c509839-509690 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T225469 NZ_CP121151:4197352-4197630 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT225469 NZ_CP087564:509468-509646 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin