Detailed information of TA system TA225463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3686470..3687180
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C6DCB3
• Locus tag: LOZ86_RS16580
• Protein ID: WP_015841346.1
• Coordinates: 3686866..3687180 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOZ86_RS16575
• Protein ID: WP_039512487.1
• Coordinates: 3686470..3686811 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS16525 (LOZ86_16525)	3681721..3682848	+	1128	WP_230242416.1	phage major capsid protein, P2 family	-
LOZ86_RS16530 (LOZ86_16530)	3682852..3683511	+	660	WP_230242418.1	terminase endonuclease subunit	-
LOZ86_RS16535 (LOZ86_16535)	3683603..3684106	+	504	WP_230242420.1	head completion/stabilization protein	-
LOZ86_RS16540 (LOZ86_16540)	3684106..3684309	+	204	WP_230242422.1	tail protein X	-
LOZ86_RS16545 (LOZ86_16545)	3684300..3684521	+	222	WP_033071244.1	HP1 family phage holin	-
LOZ86_RS16550 (LOZ86_16550)	3684505..3685014	+	510	WP_230242423.1	lysozyme	-
LOZ86_RS16555 (LOZ86_16555)	3685011..3685370	+	360	Protein_3225	Rz-like lysis system protein LysB	-
LOZ86_RS16560 (LOZ86_16560)	3685291..3685581	+	291	WP_230242431.1	Rz1-like lysis system protein LysC	-
LOZ86_RS16565 (LOZ86_16565)	3685544..3685999	+	456	WP_230242445.1	phage tail protein	-
LOZ86_RS16570 (LOZ86_16570)	3685992..3686441	+	450	WP_230242447.1	phage virion morphogenesis protein	-
LOZ86_RS16575 (LOZ86_16575)	3686470..3686811	-	342	WP_039512487.1	HigA family addiction module antitoxin	Antitoxin
LOZ86_RS16580 (LOZ86_16580)	3686866..3687180	-	315	WP_015841346.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOZ86_RS16585 (LOZ86_16585)	3687525..3688190	+	666	WP_039512486.1	ORF6N domain-containing protein	-
LOZ86_RS16590 (LOZ86_16590)	3688232..3689284	-	1053	WP_230242448.1	hypothetical protein	-
LOZ86_RS16595 (LOZ86_16595)	3689387..3690022	+	636	WP_230242450.1	phage baseplate assembly protein V	-
LOZ86_RS16600 (LOZ86_16600)	3690019..3690363	+	345	WP_039512482.1	GPW/gp25 family protein	-
LOZ86_RS16605 (LOZ86_16605)	3690367..3691275	+	909	WP_161546603.1	baseplate assembly protein	-
LOZ86_RS16610 (LOZ86_16610)	3691268..3691879	+	612	WP_161546604.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	3662970..3708255	45285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12228.00 Da
• Isoelectric Point: 9.9719

>T225463 WP_015841346.1 NZ_CP087392:c3687180-3686866 [Pectobacterium parvum]
MQKMRSIHSFRDQWLEDFFMYGKSHKKIPADVVTSLSRKLDIINAAVSAKDLRSPPGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAEDIYLDDHGYKKHK

Nucleotide

• Length: 315 bp

>T225463 NZ_CP121151:3042658-3042765 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12958.54 Da
• Isoelectric Point: 4.3501

>AT225463 WP_039512487.1 NZ_CP087392:c3686811-3686470 [Pectobacterium parvum]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLEDFLAQRDENVKDVS

Nucleotide

• Length: 342 bp

>AT225463 NZ_CP121151:c3042611-3042545 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A7V8Q8Q5