Detailed information of TA system TA225451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 29703..30360
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOZ86_RS00135
• Protein ID: WP_039485180.1
• Coordinates: 29989..30360 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: LOZ86_RS00130
• Protein ID: WP_010281167.1
• Coordinates: 29703..30002 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS00110 (LOZ86_00110)	24956..25945	+	990	WP_230242745.1	nucleoside hydrolase	-
LOZ86_RS00115 (LOZ86_00115)	25945..26922	+	978	WP_010296900.1	ABC transporter permease	-
LOZ86_RS00120 (LOZ86_00120)	26919..27827	+	909	WP_039485174.1	ABC transporter permease	-
LOZ86_RS00125 (LOZ86_00125)	27867..29666	+	1800	WP_039485177.1	ABC transporter ATP-binding protein	-
LOZ86_RS00130 (LOZ86_00130)	29703..30002	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
LOZ86_RS00135 (LOZ86_00135)	29989..30360	-	372	WP_039485180.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOZ86_RS00140 (LOZ86_00140)	30698..31396	+	699	WP_230243179.1	D-galactonate utilization transcriptional regulator DgoR	-
LOZ86_RS00145 (LOZ86_00145)	31393..32289	+	897	WP_039485185.1	2-dehydro-3-deoxygalactonokinase	-
LOZ86_RS00150 (LOZ86_00150)	32273..32890	+	618	WP_111778603.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
LOZ86_RS00155 (LOZ86_00155)	32887..34035	+	1149	WP_005976723.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14144.08 Da
• Isoelectric Point: 4.8510

>T225451 WP_039485180.1 NZ_CP087392:c30360-29989 [Pectobacterium parvum]
MAWEIVTRALFDTWFDEQTEAVQEEILAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFDRTRKA
IILCAGDKKGEDEKLFYERMIKIADAEFTSHLISPEVKEDGNA

Nucleotide

• Length: 372 bp

>T225451 NZ_CP121151:263415-263645 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT225451 WP_010281167.1 NZ_CP087392:c30002-29703 [Pectobacterium parvum]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT225451 NZ_CP121151:263642-264001 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287