Detailed information of TA system TA022545

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF3/-
• Location: 2090855..2091154
• Replicon: chromosome
• Accession: NC_010079
• Organism: Staphylococcus aureus subsp. aureus USA300 TCH1516

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: USA300HOU_RS15450
• Protein ID: WP_011447039.1
• Coordinates: 2090978..2091154 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2090855..2090910 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
USA300HOU_RS10545	2086186..2086446	+	261	WP_001791826.1	hypothetical protein	-
USA300HOU_RS10550	2086499..2086849	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
USA300HOU_RS10555	2087534..2087983	+	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
USA300HOU_RS15810	2088078..2088413	-	336	Protein_2005	SH3 domain-containing protein	-
USA300HOU_RS10565	2089063..2089554	-	492	WP_000919350.1	staphylokinase	-
USA300HOU_RS10570	2089745..2090500	-	756	WP_000861038.1	CHAP domain-containing protein	-
USA300HOU_RS10575	2090512..2090766	-	255	WP_000611512.1	phage holin	-
USA300HOU_RS10580	2090818..2090925	+	108	WP_001791821.1	hypothetical protein	-
-	2090847..2090986	+	140	NuclAT_0	-	-
-	2090847..2090986	+	140	NuclAT_0	-	-
-	2090847..2090986	+	140	NuclAT_0	-	-
-	2090847..2090986	+	140	NuclAT_0	-	-
-	2090855..2090910	+	56	-	-	Antitoxin
USA300HOU_RS15450	2090978..2091154	-	177	WP_011447039.1	putative holin-like toxin	Toxin
USA300HOU_RS10590	2091304..2091600	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
USA300HOU_RS10595	2091658..2091945	-	288	WP_001040261.1	hypothetical protein	-
USA300HOU_RS10600	2091992..2092144	-	153	WP_001153681.1	hypothetical protein	-
USA300HOU_RS10605	2092134..2095919	-	3786	WP_000582165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / sak / hlb / groEL	2086499..2142447	55948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T22545 WP_011447039.1 NC_010079:c2091154-2090978 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T22545 NC_010079:c2091154-2090978 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 56 bp

>AT22545 NC_010079:2090855-2090910 [Staphylococcus aureus subsp. aureus USA300_TCH1516]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• PDB: 7NS1

Antitoxin