Detailed information of TA system TA225444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2281945..2282675
• Replicon: chromosome
• Accession: NZ_CP087390
• Organism: Enterobacter sp. AN-K1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: LOC22_RS10925
• Protein ID: WP_023302825.1
• Coordinates: 2281945..2282259 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOC22_RS10930
• Protein ID: WP_127350921.1
• Coordinates: 2282256..2282675 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOC22_RS10905 (LOC22_10905)	2277611..2278696	+	1086	Protein_2122	cellulase family glycosylhydrolase	-
LOC22_RS10910 (LOC22_10910)	2278602..2279786	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
LOC22_RS10915 (LOC22_10915)	2279962..2280795	-	834	WP_133982172.1	DMT family transporter	-
LOC22_RS10920 (LOC22_10920)	2280858..2281304	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
LOC22_RS10925 (LOC22_10925)	2281945..2282259	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
LOC22_RS10930 (LOC22_10930)	2282256..2282675	+	420	WP_127350921.1	helix-turn-helix domain-containing protein	Antitoxin
LOC22_RS10935 (LOC22_10935)	2282822..2282920	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
LOC22_RS10940 (LOC22_10940)	2283027..2284715	+	1689	WP_062938354.1	acetolactate synthase large subunit	-
LOC22_RS10945 (LOC22_10945)	2284719..2285006	+	288	WP_015570113.1	acetolactate synthase small subunit	-
LOC22_RS10950 (LOC22_10950)	2285132..2285725	+	594	WP_003861054.1	transcriptional regulator UhpA	-
LOC22_RS10955 (LOC22_10955)	2285722..2287227	+	1506	WP_033487017.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T225444 WP_023302825.1 NZ_CP087390:2281945-2282259 [Enterobacter sp. AN-K1]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T225444 NZ_CP121149:3624629-3624847 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15464.10 Da
• Isoelectric Point: 5.6761

>AT225444 WP_127350921.1 NZ_CP087390:2282256-2282675 [Enterobacter sp. AN-K1]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHFEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT225444 NZ_CP121149:3624229-3624603 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A837FIL2