Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2163335..2163972 Replicon chromosome
Accession NZ_CP087379
Organism Mannheimia haemolytica strain IMT47952

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LO774_RS11110 Protein ID WP_040080900.1
Coordinates 2163335..2163640 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LO774_RS11115 Protein ID WP_040080901.1
Coordinates 2163652..2163972 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO774_RS11070 (LO774_11070) 2158345..2159544 - 1200 WP_230269034.1 terminase -
LO774_RS11075 (LO774_11075) 2159547..2160044 - 498 WP_040080897.1 hypothetical protein -
LO774_RS11080 (LO774_11080) 2160477..2160710 - 234 WP_162839428.1 hypothetical protein -
LO774_RS11085 (LO774_11085) 2160676..2161005 - 330 WP_040080898.1 DUF2570 family protein -
LO774_RS11090 (LO774_11090) 2161006..2161602 - 597 WP_020824120.1 glycoside hydrolase family 19 protein -
LO774_RS11095 (LO774_11095) 2161617..2161976 - 360 WP_165876904.1 phage holin, lambda family -
LO774_RS11100 (LO774_11100) 2162112..2162585 - 474 WP_006251969.1 antiterminator Q family protein -
LO774_RS11105 (LO774_11105) 2162575..2163144 - 570 WP_040080899.1 recombination protein NinG -
LO774_RS11110 (LO774_11110) 2163335..2163640 + 306 WP_040080900.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LO774_RS11115 (LO774_11115) 2163652..2163972 + 321 WP_040080901.1 HigA family addiction module antitoxin Antitoxin
LO774_RS11120 (LO774_11120) 2164039..2164497 + 459 WP_040080902.1 hypothetical protein -
LO774_RS11125 (LO774_11125) 2164551..2165590 - 1040 Protein_2158 IS481 family transposase -
LO774_RS11130 (LO774_11130) 2165725..2166186 - 462 WP_020824123.1 DUF1367 family protein -
LO774_RS11135 (LO774_11135) 2166207..2166809 - 603 WP_040080903.1 DNA N-6-adenine-methyltransferase -
LO774_RS11140 (LO774_11140) 2166806..2167582 - 777 WP_230269035.1 replication protein P -
LO774_RS11145 (LO774_11145) 2167640..2167984 - 345 WP_230269036.1 hypothetical protein -
LO774_RS11150 (LO774_11150) 2167987..2168223 - 237 WP_230269037.1 hypothetical protein -
LO774_RS11155 (LO774_11155) 2168220..2168402 - 183 WP_230269038.1 helix-turn-helix domain-containing protein -
LO774_RS11160 (LO774_11160) 2168517..2168960 - 444 WP_006252074.1 YmfL family putative regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2140938..2189523 48585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(8-97)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(4-82)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11995.61 Da        Isoelectric Point: 6.4692

>T225428 WP_040080900.1 NZ_CP087379:2163335-2163640 [Mannheimia haemolytica]
MFNLTEAHFRDEALYRFFQYGEVSRTIPANLTSVLARKLDMINAAEKLNDLRSPPANRLELLEPKQNNVYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL

Download         Length: 306 bp

>T225428 NZ_CP121147:c4706013-4705639 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 12089.80 Da        Isoelectric Point: 6.2152

>AT225428 WP_040080901.1 NZ_CP087379:2163652-2163972 [Mannheimia haemolytica]
MMQRKPTPVGEILQEEFLAPLNLKIGDLVEILDVHRNTASNLVNNSCRVSPEMAFKLAKAFDTSPEFWLNLQTATDLWEL
RHNNRFQQSLDSVKAVNHWQGVPAFA

Download         Length: 321 bp

>AT225428 NZ_CP121147:c4706255-4706013 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References