Detailed information of TA system TA225419

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119366..119792
• Replicon: plasmid pRIVM_C019217_2
• Accession: NZ_CP087378
• Organism: Escherichia coli strain RIVM_C019217

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LN359_RS26100
• Protein ID: WP_001372321.1
• Coordinates: 119366..119491 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	119568..119792 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN359_RS26060 (114405)	114405..114632	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
LN359_RS26065 (114726)	114726..115412	-	687	WP_000332487.1	PAS domain-containing protein	-
LN359_RS26070 (115606)	115606..115989	-	384	WP_001151564.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LN359_RS26075 (116275)	116275..116922	+	648	WP_124760006.1	transglycosylase SLT domain-containing protein	-
LN359_RS26080 (117219)	117219..118040	-	822	WP_001234469.1	DUF932 domain-containing protein	-
LN359_RS26085 (118160)	118160..118447	-	288	WP_000107537.1	hypothetical protein	-
LN359_RS26090 (118745)	118745..118918	+	174	Protein_145	hypothetical protein	-
LN359_RS26095 (118916)	118916..119146	-	231	WP_071587244.1	hypothetical protein	-
LN359_RS26100 (119366)	119366..119491	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LN359_RS26105 (119433)	119433..119582	-	150	Protein_148	plasmid maintenance protein Mok	-
- (119568)	119568..119792	-	225	NuclAT_0	-	Antitoxin
- (119568)	119568..119792	-	225	NuclAT_0	-	Antitoxin
- (119568)	119568..119792	-	225	NuclAT_0	-	Antitoxin
- (119568)	119568..119792	-	225	NuclAT_0	-	Antitoxin
LN359_RS26110 (119604)	119604..119792	+	189	WP_001299721.1	hypothetical protein	-
LN359_RS26115 (119761)	119761..120523	-	763	Protein_150	plasmid SOS inhibition protein A	-
LN359_RS26120 (120520)	120520..120954	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
LN359_RS26125 (121009)	121009..122967	-	1959	WP_229327587.1	ParB/RepB/Spo0J family partition protein	-
LN359_RS26130 (123026)	123026..123259	-	234	WP_000006018.1	DUF905 domain-containing protein	-
LN359_RS26135 (123315)	123315..123842	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
LN359_RS26140 (124312)	124312..124560	-	249	WP_071606928.1	hypothetical protein	-
LN359_RS26145 (124482)	124482..124712	-	231	WP_001333093.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..147403	147403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225419 WP_001372321.1 NZ_CP087378:c119491-119366 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225419 NZ_CP121147:c2755895-2755793 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 225 bp

>AT225419 NZ_CP087378:c119792-119568 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin