Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5027557..5028159 Replicon chromosome
Accession NZ_CP087377
Organism Escherichia coli strain RIVM_C019217

Toxin (Protein)


Gene name higB Uniprot ID A0A4Y9BEM6
Locus tag LN359_RS24405 Protein ID WP_059332311.1
Coordinates 5027848..5028159 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LN359_RS24400 Protein ID WP_000356395.1
Coordinates 5027557..5027847 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN359_RS24365 (5023181) 5023181..5024083 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
LN359_RS24370 (5024080) 5024080..5024715 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LN359_RS24375 (5024712) 5024712..5025641 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
LN359_RS24380 (5025823) 5025823..5026065 - 243 WP_001309881.1 CopG family transcriptional regulator -
LN359_RS24385 (5026284) 5026284..5026502 - 219 WP_001295676.1 CopG family transcriptional regulator -
LN359_RS24390 (5026921) 5026921..5027199 - 279 WP_001315112.1 hypothetical protein -
LN359_RS24395 (5027251) 5027251..5027472 - 222 WP_001550354.1 hypothetical protein -
LN359_RS24400 (5027557) 5027557..5027847 - 291 WP_000356395.1 NadS family protein Antitoxin
LN359_RS24405 (5027848) 5027848..5028159 - 312 WP_059332311.1 hypothetical protein Toxin
LN359_RS24410 (5028388) 5028388..5029296 + 909 WP_001550353.1 alpha/beta hydrolase -
LN359_RS24415 (5029464) 5029464..5030378 - 915 WP_109553727.1 transposase -
LN359_RS24420 (5030391) 5030391..5031278 - 888 Protein_4778 hypothetical protein -
LN359_RS24425 (5031694) 5031694..5032635 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
LN359_RS24430 (5032680) 5032680..5033117 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12212.25 Da        Isoelectric Point: 10.0241

>T225417 WP_059332311.1 NZ_CP087377:c5028159-5027848 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T225417 NZ_CP121147:c2155063-2154956 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT225417 WP_000356395.1 NZ_CP087377:c5027847-5027557 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT225417 NZ_CP121147:2155110-2155176 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y9BEM6

Antitoxin

Source ID Structure

References