Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 675798..676597 Replicon chromosome
Accession NZ_CP087377
Organism Escherichia coli strain RIVM_C019217

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag LN359_RS03275 Protein ID WP_000347270.1
Coordinates 675798..676262 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag LN359_RS03280 Protein ID WP_001551693.1
Coordinates 676262..676597 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN359_RS03245 (670799) 670799..671233 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LN359_RS03250 (671251) 671251..672129 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LN359_RS03255 (672119) 672119..672898 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LN359_RS03260 (672909) 672909..673382 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LN359_RS03265 (673405) 673405..674685 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
LN359_RS03270 (674934) 674934..675743 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LN359_RS03275 (675798) 675798..676262 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LN359_RS03280 (676262) 676262..676597 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LN359_RS03285 (676746) 676746..678317 - 1572 WP_001273738.1 galactarate dehydratase -
LN359_RS03290 (678692) 678692..680026 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LN359_RS03295 (680042) 680042..680812 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T225393 WP_000347270.1 NZ_CP087377:c676262-675798 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T225393 NZ_CP121145:81579-81962 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT225393 WP_001551693.1 NZ_CP087377:c676597-676262 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT225393 NZ_CP121145:81337-81579 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References