Detailed information of TA system TA225384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 52362..53020
• Replicon: plasmid p1DB006
• Accession: NZ_CP087349
• Organism: Acinetobacter baumannii strain DB006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LO006_RS19410
• Protein ID: WP_000312250.1
• Coordinates: 52661..53020 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO006_RS19405
• Protein ID: WP_001096429.1
• Coordinates: 52362..52661 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO006_RS19365	47459..48427	-	969	WP_012308653.1	IS30 family transposase	-
LO006_RS19370	48773..49030	+	258	WP_000834290.1	hypothetical protein	-
LO006_RS19375	49035..49607	+	573	WP_000443897.1	hypothetical protein	-
LO006_RS19380	49583..49762	+	180	WP_000387630.1	hypothetical protein	-
LO006_RS19385	49779..50408	+	630	WP_000701003.1	hypothetical protein	-
LO006_RS19390	50448..50666	+	219	WP_001043201.1	hypothetical protein	-
LO006_RS19395	50686..51240	+	555	WP_000790084.1	hypothetical protein	-
LO006_RS19400	51290..51826	+	537	WP_057694987.1	hypothetical protein	-
LO006_RS19405	52362..52661	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LO006_RS19410	52661..53020	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO006_RS19415	53221..53787	+	567	WP_000710385.1	hypothetical protein	-
LO006_RS19420	53836..54018	+	183	WP_000373385.1	hypothetical protein	-
LO006_RS19425	54085..54783	+	699	WP_000873188.1	hypothetical protein	-
LO006_RS19430	54922..55305	+	384	WP_000654348.1	hypothetical protein	-
LO006_RS19435	55372..56130	+	759	WP_001053127.1	hypothetical protein	-
LO006_RS19440	57185..57499	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..71410	71410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T225384 WP_000312250.1 NZ_CP087349:c53020-52661 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T225384 NZ_CP121143:c3429112-3428579 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTAGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT225384 WP_001096429.1 NZ_CP087349:c52661-52362 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT225384 NZ_CP121143:c3429389-3429123 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA