Detailed information of TA system TA225381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 49930..50588
• Replicon: plasmid p1DB007
• Accession: NZ_CP087345
• Organism: Acinetobacter baumannii strain DB007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOZ99_RS20460
• Protein ID: WP_000312250.1
• Coordinates: 50229..50588 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOZ99_RS20455
• Protein ID: WP_001096429.1
• Coordinates: 49930..50229 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ99_RS20415	45027..45995	-	969	WP_012308653.1	IS30 family transposase	-
LOZ99_RS20420	46341..46598	+	258	WP_000834290.1	hypothetical protein	-
LOZ99_RS20425	46603..47175	+	573	WP_000443897.1	hypothetical protein	-
LOZ99_RS20430	47151..47330	+	180	WP_000387630.1	hypothetical protein	-
LOZ99_RS20435	47347..47976	+	630	WP_000701003.1	hypothetical protein	-
LOZ99_RS20440	48016..48234	+	219	WP_001043201.1	hypothetical protein	-
LOZ99_RS20445	48254..48808	+	555	WP_000790084.1	hypothetical protein	-
LOZ99_RS20450	48858..49394	+	537	WP_057694987.1	hypothetical protein	-
LOZ99_RS20455	49930..50229	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LOZ99_RS20460	50229..50588	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOZ99_RS20465	50789..51355	+	567	WP_000710385.1	hypothetical protein	-
LOZ99_RS20470	51404..51586	+	183	WP_000373385.1	hypothetical protein	-
LOZ99_RS20475	51653..52351	+	699	WP_000873188.1	hypothetical protein	-
LOZ99_RS20480	52490..52873	+	384	WP_000654348.1	hypothetical protein	-
LOZ99_RS20485	52940..53698	+	759	WP_001053127.1	hypothetical protein	-
LOZ99_RS20490	54753..55067	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..70098	70098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T225381 WP_000312250.1 NZ_CP087345:c50588-50229 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T225381 NZ_CP121143:754514-754616 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT225381 WP_001096429.1 NZ_CP087345:c50229-49930 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT225381 NZ_CP121143:c754623-754478 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT