Detailed information of TA system TA225373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 24079..24664
• Replicon: plasmid p3DB053
• Accession: NZ_CP087338
• Organism: Acinetobacter baumannii strain DB053

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9L5H5
• Locus tag: LO010_RS20675
• Protein ID: WP_005175528.1
• Coordinates: 24344..24664 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO010_RS20670
• Protein ID: WP_004896911.1
• Coordinates: 24079..24351 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO010_RS20645	19366..19641	+	276	WP_001133624.1	metal/formaldehyde-sensitive transcriptional repressor	-
LO010_RS20650	19663..20775	+	1113	WP_004897268.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
LO010_RS20655	21004..21834	+	831	WP_004897267.1	S-formylglutathione hydrolase	-
LO010_RS20660	22168..22470	+	303	WP_004897265.1	hypothetical protein	-
LO010_RS20665	22561..23265	-	705	WP_001067783.1	IS6-like element IS1008 family transposase	-
LO010_RS20670	24079..24351	-	273	WP_004896911.1	NadS family protein	Antitoxin
LO010_RS20675	24344..24664	-	321	WP_005175528.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO010_RS20680	24786..25220	-	435	WP_258580557.1	hypothetical protein	-
LO010_RS20685	25579..25725	-	147	WP_167850448.1	hypothetical protein	-
LO010_RS20690	25883..26425	-	543	WP_005175521.1	ImmA/IrrE family metallo-endopeptidase	-
LO010_RS20695	26435..26788	-	354	WP_005175518.1	helix-turn-helix transcriptional regulator	-
LO010_RS20700	27078..27782	-	705	WP_258580558.1	IS6-like element IS1008 family transposase	-
LO010_RS20705	27768..28301	+	534	WP_258580559.1	primase C-terminal domain-containing protein	-
LO010_RS20710	28471..28660	-	190	Protein_33	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32159	32159
	-	inside	IScluster/Tn	-	-	2816..27782	24966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12248.09 Da
• Isoelectric Point: 7.9835

>T225373 WP_005175528.1 NZ_CP087338:c24664-24344 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVNDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKEISILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T225373 NZ_CP121141:1296547-1296930 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10126.59 Da
• Isoelectric Point: 7.2045

>AT225373 WP_004896911.1 NZ_CP087338:c24351-24079 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEMKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT225373 NZ_CP121141:1296305-1296547 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: N9L5H5