Detailed information of TA system TA225366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22398..23049
• Replicon: plasmid p1OC074
• Accession: NZ_CP087329
• Organism: Acinetobacter baumannii strain OC074

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: LOZ98_RS19075
• Protein ID: WP_000269904.1
• Coordinates: 22693..23049 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOZ98_RS19070
• Protein ID: WP_024436604.1
• Coordinates: 22398..22700 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ98_RS19040	19130..19834	+	705	WP_004741406.1	IS6-like element IS1006 family transposase	-
LOZ98_RS19045	19889..20116	+	228	Protein_23	recombinase family protein	-
LOZ98_RS19050	20341..20769	-	429	WP_016653412.1	hypothetical protein	-
LOZ98_RS19055	20952..21464	-	513	WP_024436968.1	SMI1/KNR4 family protein	-
LOZ98_RS19060	21503..22021	-	519	WP_000447196.1	tetratricopeptide repeat protein	-
LOZ98_RS19065	22196..22405	-	210	WP_000069475.1	hypothetical protein	-
LOZ98_RS19070	22398..22700	-	303	WP_024436604.1	XRE family transcriptional regulator	Antitoxin
LOZ98_RS19075	22693..23049	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOZ98_RS19080	23766..25820	+	2055	WP_004740259.1	TonB-dependent receptor	-
LOZ98_RS19085	25934..26620	+	687	WP_004740261.1	DUF4145 domain-containing protein	-
LOZ98_RS19090	26662..26769	+	108	Protein_32	transcriptional regulator	-
LOZ98_RS19095	26967..27302	+	336	WP_004740263.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..88290	88290
	-	inside	IScluster/Tn	-	-	27570..40497	12927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T225366 WP_000269904.1 NZ_CP087329:c23049-22693 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T225366 NZ_CP121139:c4825484-4824999 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATGCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11181.00 Da
• Isoelectric Point: 7.3198

>AT225366 WP_024436604.1 NZ_CP087329:c22700-22398 [Acinetobacter baumannii]
MAKSLQELLASRSPESQTRIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT225366 NZ_CP121139:c4825738-4825472 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: N8YFZ1