Detailed information of TA system TA225360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 83623..84274
• Replicon: plasmid p1OC073
• Accession: NZ_CP087326
• Organism: Acinetobacter baumannii strain OC073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: LO013_RS18740
• Protein ID: WP_000269904.1
• Coordinates: 83623..83979 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO013_RS18745
• Protein ID: WP_024436604.1
• Coordinates: 83972..84274 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO013_RS18720	79370..79705	-	336	WP_004740263.1	carboxymuconolactone decarboxylase family protein	-
LO013_RS18725	79903..80010	-	108	Protein_80	transcriptional regulator	-
LO013_RS18730	80052..80774	-	723	WP_227555798.1	DUF4145 domain-containing protein	-
LO013_RS18735	80852..82906	-	2055	WP_004740259.1	TonB-dependent receptor	-
LO013_RS18740	83623..83979	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO013_RS18745	83972..84274	+	303	WP_024436604.1	XRE family transcriptional regulator	Antitoxin
LO013_RS18750	84267..84476	+	210	WP_000069475.1	hypothetical protein	-
LO013_RS18755	84651..85169	+	519	WP_000447196.1	tetratricopeptide repeat protein	-
LO013_RS18760	85208..85720	+	513	WP_024436968.1	SMI1/KNR4 family protein	-
LO013_RS18765	85903..86331	+	429	WP_016653412.1	hypothetical protein	-
LO013_RS18770	86490..87824	+	1335	WP_032050497.1	ISNCY family transposase	-
LO013_RS18775	88161..88457	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LO013_RS18780	88459..88749	+	291	WP_000241337.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113509	113509
	-	inside	IScluster/Tn	-	-	86502..98478	11976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T225360 WP_000269904.1 NZ_CP087326:83623-83979 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T225360 NZ_CP121139:c993951-993733 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11181.00 Da
• Isoelectric Point: 7.3198

>AT225360 WP_024436604.1 NZ_CP087326:83972-84274 [Acinetobacter baumannii]
MAKSLQELLASRSPESQTRIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT225360 NZ_CP121139:c994352-993978 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: N8YFZ1