Detailed information of TA system TA225345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7686..8344
• Replicon: plasmid p2OC059
• Accession: NZ_CP087314
• Organism: Acinetobacter baumannii strain OC059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LO007_RS19450
• Protein ID: WP_000312250.1
• Coordinates: 7686..8045 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO007_RS19455
• Protein ID: WP_001096429.1
• Coordinates: 8045..8344 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO007_RS19420	3207..3521	-	315	WP_000708714.1	hypothetical protein	-
LO007_RS19425	4576..5334	-	759	WP_001053127.1	hypothetical protein	-
LO007_RS19430	5401..5784	-	384	WP_000654348.1	hypothetical protein	-
LO007_RS19435	5923..6621	-	699	WP_000873188.1	hypothetical protein	-
LO007_RS19440	6688..6870	-	183	WP_000373385.1	hypothetical protein	-
LO007_RS19445	6919..7485	-	567	WP_000710385.1	hypothetical protein	-
LO007_RS19450	7686..8045	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO007_RS19455	8045..8344	+	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LO007_RS19460	8882..9418	-	537	WP_000731978.1	hypothetical protein	-
LO007_RS19465	9468..10022	-	555	WP_000790084.1	hypothetical protein	-
LO007_RS19470	10042..10260	-	219	WP_001043201.1	hypothetical protein	-
LO007_RS19475	10300..10929	-	630	WP_000701003.1	hypothetical protein	-
LO007_RS19480	10946..11125	-	180	WP_000387630.1	hypothetical protein	-
LO007_RS19485	11101..11673	-	573	WP_000443897.1	hypothetical protein	-
LO007_RS19490	11678..11935	-	258	WP_000834290.1	hypothetical protein	-
LO007_RS19495	12040..13320	-	1281	WP_001093570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..70100	70100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T225345 WP_000312250.1 NZ_CP087314:7686-8045 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T225345 NZ_CP121137:754508-754610 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT225345 WP_001096429.1 NZ_CP087314:8045-8344 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT225345 NZ_CP121137:c754617-754472 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT