Detailed information of TA system TA225344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 36176..36761
• Replicon: plasmid p1OC059
• Accession: NZ_CP087313
• Organism: Acinetobacter baumannii strain OC059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: LO007_RS18425
• Protein ID: WP_000897311.1
• Coordinates: 36176..36496 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO007_RS18430
• Protein ID: WP_000369784.1
• Coordinates: 36489..36761 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO007_RS18405	31983..32312	+	330	WP_000506885.1	DUF4236 domain-containing protein	-
LO007_RS18410	32584..33816	-	1233	WP_001255535.1	ATP-binding protein	-
LO007_RS18415	34074..34511	-	438	Protein_26	IS3 family transposase	-
LO007_RS18420	34565..35497	+	933	WP_000743272.1	IS5-like element IS17 family transposase	-
LO007_RS18425	36176..36496	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO007_RS18430	36489..36761	+	273	WP_000369784.1	NadS family protein	Antitoxin
LO007_RS18435	36794..37594	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
LO007_RS18440	37685..37986	+	302	Protein_31	IS3 family transposase	-
LO007_RS18445	38102..38314	+	213	WP_001123161.1	hypothetical protein	-
LO007_RS18450	38640..39497	-	858	WP_000609033.1	LysR family transcriptional regulator	-
LO007_RS18455	39494..40501	-	1008	WP_005071472.1	phosphonate dehydrogenase	-
LO007_RS18460	40543..41340	-	798	WP_004757464.1	phosphonate ABC transporter, permease protein PhnE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(B) / aph(6)-Id / aph(3'')-Ib	-	1..223334	223334
	-	inside	IScluster/Tn	-	-	34074..38020	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T225344 WP_000897311.1 NZ_CP087313:36176-36496 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T225344 NZ_CP121136:158724-159206 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT225344 WP_000369784.1 NZ_CP087313:36489-36761 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT225344 NZ_CP121136:158470-158736 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A7S7BY50