Detailed information of TA system TA225341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 53603..54261
• Replicon: plasmid p2OC068
• Accession: NZ_CP087311
• Organism: Acinetobacter baumannii strain OC068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LO005_RS19370
• Protein ID: WP_000312250.1
• Coordinates: 53902..54261 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO005_RS19365
• Protein ID: WP_001096429.1
• Coordinates: 53603..53902 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO005_RS19325	48627..49907	+	1281	WP_001093570.1	hypothetical protein	-
LO005_RS19330	50012..50269	+	258	WP_000834290.1	hypothetical protein	-
LO005_RS19335	50274..50846	+	573	WP_000443897.1	hypothetical protein	-
LO005_RS19340	50822..51001	+	180	WP_000387630.1	hypothetical protein	-
LO005_RS19345	51018..51647	+	630	WP_000701003.1	hypothetical protein	-
LO005_RS19350	51687..51905	+	219	WP_001043201.1	hypothetical protein	-
LO005_RS19355	51925..52479	+	555	WP_000790084.1	hypothetical protein	-
LO005_RS19360	52529..53065	+	537	WP_000731978.1	hypothetical protein	-
LO005_RS19365	53603..53902	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LO005_RS19370	53902..54261	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO005_RS19375	54462..55028	+	567	WP_000710385.1	hypothetical protein	-
LO005_RS19380	55077..55259	+	183	WP_000373385.1	hypothetical protein	-
LO005_RS19385	55326..56024	+	699	WP_000873188.1	hypothetical protein	-
LO005_RS19390	56163..56546	+	384	WP_000654348.1	hypothetical protein	-
LO005_RS19395	56613..57116	+	504	WP_001053125.1	hypothetical protein	-
LO005_RS19400	57533..57940	+	408	Protein_76	Mu transposase C-terminal domain-containing protein	-
LO005_RS19405	57943..58935	+	993	WP_001381192.1	TniB family NTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / sul1 / qacE / dfrA7 / aac(6')-Ib / blaGES-11 / aph(6)-Id / aph(3'')-Ib / aadA2 / cmlA1 / ant(2'')-Ia	-	1..83611	83611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T225341 WP_000312250.1 NZ_CP087311:c54261-53902 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T225341 NZ_CP121135:c2504771-2504553 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT225341 WP_001096429.1 NZ_CP087311:c53902-53603 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT225341 NZ_CP121135:c2505172-2504798 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG