Detailed information of TA system TA225334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 37801..38459
• Replicon: plasmid p1OC070
• Accession: NZ_CP087299
• Organism: Acinetobacter baumannii strain OC070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LO009_RS19075
• Protein ID: WP_000312250.1
• Coordinates: 37801..38160 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO009_RS19080
• Protein ID: WP_001096429.1
• Coordinates: 38160..38459 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO009_RS19040	33127..34119	-	993	WP_001381192.1	TniB family NTP-binding protein	-
LO009_RS19045	34122..34529	-	408	Protein_42	Mu transposase C-terminal domain-containing protein	-
LO009_RS19050	34946..35449	-	504	WP_001053125.1	hypothetical protein	-
LO009_RS19055	35516..35899	-	384	WP_000654348.1	hypothetical protein	-
LO009_RS19060	36038..36736	-	699	WP_000873188.1	hypothetical protein	-
LO009_RS19065	36803..36985	-	183	WP_000373385.1	hypothetical protein	-
LO009_RS19070	37034..37600	-	567	WP_000710385.1	hypothetical protein	-
LO009_RS19075	37801..38160	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO009_RS19080	38160..38459	+	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
LO009_RS19085	38997..39533	-	537	WP_000731978.1	hypothetical protein	-
LO009_RS19090	39583..40137	-	555	WP_000790084.1	hypothetical protein	-
LO009_RS19095	40157..40375	-	219	WP_001043201.1	hypothetical protein	-
LO009_RS19100	40415..41044	-	630	WP_000701003.1	hypothetical protein	-
LO009_RS19105	41061..41240	-	180	WP_000387630.1	hypothetical protein	-
LO009_RS19110	41216..41788	-	573	WP_000443897.1	hypothetical protein	-
LO009_RS19115	41793..42050	-	258	WP_000834290.1	hypothetical protein	-
LO009_RS19120	42155..43435	-	1281	WP_001093570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / ant(2'')-Ia / cmlA1 / aadA2 / aph(3'')-Ib / aph(6)-Id / blaGES-14 / aac(6')-Ib / dfrA7 / qacE / sul1	-	1..83611	83611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T225334 WP_000312250.1 NZ_CP087299:37801-38160 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T225334 NZ_CP121135:c866112-865765 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAATCGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT225334 WP_001096429.1 NZ_CP087299:38160-38459 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT225334 NZ_CP121135:c865765-865466 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA