Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4856838..4857440 Replicon chromosome
Accession NZ_CP087294
Organism Escherichia coli strain APEC E956

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag LO740_RS23540 Protein ID WP_000897305.1
Coordinates 4856838..4857149 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LO740_RS23545 Protein ID WP_000356397.1
Coordinates 4857150..4857440 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO740_RS23515 (4852752) 4852752..4853351 + 600 WP_001315111.1 glucose-1-phosphatase -
LO740_RS23520 (4853345) 4853345..4854217 + 873 WP_000920762.1 virulence factor BrkB family protein -
LO740_RS23525 (4854214) 4854214..4854651 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
LO740_RS23530 (4854696) 4854696..4855637 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
LO740_RS23535 (4855701) 4855701..4856609 - 909 WP_001318161.1 alpha/beta hydrolase -
LO740_RS23540 (4856838) 4856838..4857149 + 312 WP_000897305.1 hypothetical protein Toxin
LO740_RS23545 (4857150) 4857150..4857440 + 291 WP_000356397.1 NadS family protein Antitoxin
LO740_RS23550 (4858045) 4858045..4858263 + 219 WP_001295676.1 CopG family transcriptional regulator -
LO740_RS23555 (4858478) 4858478..4859407 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
LO740_RS23560 (4859404) 4859404..4860039 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LO740_RS23565 (4860036) 4860036..4860938 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T225329 WP_000897305.1 NZ_CP087294:4856838-4857149 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T225329 NZ_CP121133:c4916748-4916401 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAATCGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT225329 WP_000356397.1 NZ_CP087294:4857150-4857440 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT225329 NZ_CP121133:c4916401-4916102 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References