Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4033313..4034112 Replicon chromosome
Accession NZ_CP087294
Organism Escherichia coli strain APEC E956

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LO740_RS19530 Protein ID WP_000347251.1
Coordinates 4033648..4034112 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag LO740_RS19525 Protein ID WP_001296435.1
Coordinates 4033313..4033648 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO740_RS19510 (4029098) 4029098..4029868 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
LO740_RS19515 (4029884) 4029884..4031218 - 1335 WP_274491318.1 galactarate/glucarate/glycerate transporter GarP -
LO740_RS19520 (4031593) 4031593..4033164 + 1572 WP_001273941.1 galactarate dehydratase -
LO740_RS19525 (4033313) 4033313..4033648 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LO740_RS19530 (4033648) 4033648..4034112 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LO740_RS19535 (4034167) 4034167..4034976 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LO740_RS19540 (4035225) 4035225..4036505 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
LO740_RS19545 (4036528) 4036528..4037001 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LO740_RS19550 (4037012) 4037012..4037791 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LO740_RS19555 (4037781) 4037781..4038659 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LO740_RS19560 (4038677) 4038677..4039111 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T225320 WP_000347251.1 NZ_CP087294:4033648-4034112 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T225320 NZ_CP121132:c90514-90032 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT225320 WP_001296435.1 NZ_CP087294:4033313-4033648 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT225320 NZ_CP121132:c90768-90502 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References