Detailed information of TA system TA022532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1350361..1351032
• Replicon: chromosome
• Accession: NC_010002
• Organism: Delftia acidovorans SPH-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A543BG95
• Locus tag: DACI_RS06140
• Protein ID: WP_012203142.1
• Coordinates: 1350751..1351032 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: DACI_RS06135
• Protein ID: WP_012203141.1
• Coordinates: 1350361..1350672 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DACI_RS06125	1345861..1346874	+	1014	WP_012203140.1	type I-F CRISPR-associated endonuclease Cas1	-
DACI_RS06130	1346871..1350357	+	3487	Protein_1223	type I-F CRISPR-associated helicase Cas3	-
DACI_RS06135	1350361..1350672	-	312	WP_012203141.1	HigA family addiction module antidote protein	Antitoxin
DACI_RS06140	1350751..1351032	-	282	WP_012203142.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DACI_RS06145	1351203..1352600	+	1398	WP_012203143.1	type I-F CRISPR-associated protein Csy1	-
DACI_RS06150	1352597..1353685	+	1089	WP_012203144.1	type I-F CRISPR-associated protein Csy2	-
DACI_RS06155	1353740..1354768	+	1029	WP_012203145.1	type I-F CRISPR-associated protein Csy3	-
DACI_RS06160	1354774..1355340	+	567	WP_043783206.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10679.22 Da
• Isoelectric Point: 8.5442

>T22532 WP_012203142.1 NC_010002:c1351032-1350751 [Delftia acidovorans SPH-1]
MAITSFKCPETQRLFTSGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRVCFVWT
EKGVADVEIVDYH

Nucleotide

• Length: 282 bp

>T22532 NC_010002:c1351032-1350751 [Delftia acidovorans SPH-1]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACCCAGCGCCTGTTCACCAGCGGCAAGACCCGCCGGTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAGTTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCGTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGCTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT22532 WP_012203141.1 NC_010002:c1350672-1350361 [Delftia acidovorans SPH-1]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT22532 NC_010002:c1350672-1350361 [Delftia acidovorans SPH-1]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTCTTTGGGGGCGACGCCCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A543BG95