Detailed information of TA system TA225296

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133010..133436
• Replicon: plasmid p102026_1
• Accession: NZ_CP087291
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LO741_RS26350
• Protein ID: WP_001372321.1
• Coordinates: 133010..133135 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	133212..133436 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS26310 (128380)	128380..129069	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
LO741_RS26315 (129256)	129256..129639	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LO741_RS26320 (129961)	129961..130563	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
LO741_RS26325 (130860)	130860..131681	-	822	WP_001234469.1	DUF932 domain-containing protein	-
LO741_RS26330 (131801)	131801..132088	-	288	WP_000107535.1	hypothetical protein	-
LO741_RS26335 (132113)	132113..132319	-	207	WP_000547939.1	hypothetical protein	-
LO741_RS26340 (132389)	132389..132562	+	174	Protein_135	hypothetical protein	-
LO741_RS26345 (132560)	132560..132790	-	231	WP_001426396.1	hypothetical protein	-
LO741_RS26350 (133010)	133010..133135	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LO741_RS26355 (133077)	133077..133226	-	150	Protein_138	plasmid maintenance protein Mok	-
- (133212)	133212..133436	-	225	NuclAT_0	-	Antitoxin
- (133212)	133212..133436	-	225	NuclAT_0	-	Antitoxin
- (133212)	133212..133436	-	225	NuclAT_0	-	Antitoxin
- (133212)	133212..133436	-	225	NuclAT_0	-	Antitoxin
LO741_RS26360 (133248)	133248..133436	+	189	WP_001299721.1	hypothetical protein	-
LO741_RS26365 (133405)	133405..134167	-	763	Protein_140	plasmid SOS inhibition protein A	-
LO741_RS26370 (134164)	134164..134598	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
LO741_RS26375 (134653)	134653..136611	-	1959	WP_001145470.1	ParB/RepB/Spo0J family partition protein	-
LO741_RS26380 (136670)	136670..136903	-	234	WP_000006004.1	DUF905 domain-containing protein	-
LO741_RS26385 (136959)	136959..137444	-	486	WP_042029316.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..174489	174489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T225296 WP_001372321.1 NZ_CP087291:c133135-133010 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T225296 NZ_CP121128:195544-196026 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT225296 NZ_CP087291:c133436-133212 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin