Detailed information of TA system TA225291

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92066..92320
• Replicon: plasmid p102026_1
• Accession: NZ_CP087291
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LO741_RS26105
• Protein ID: WP_001312851.1
• Coordinates: 92066..92215 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	92259..92320 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS26070 (88396)	88396..88683	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LO741_RS26075 (88680)	88680..88931	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LO741_RS26080 (89894)	89894..90751	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LO741_RS26085 (90744)	90744..91226	-	483	WP_001273588.1	hypothetical protein	-
LO741_RS26090 (91219)	91219..91266	-	48	WP_229471593.1	hypothetical protein	-
LO741_RS26095 (91257)	91257..91508	+	252	WP_223195197.1	replication protein RepA	-
LO741_RS26100 (91525)	91525..91782	-	258	WP_000083845.1	replication regulatory protein RepA	-
LO741_RS26105 (92066)	92066..92215	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (92259)	92259..92320	+	62	NuclAT_1	-	Antitoxin
- (92259)	92259..92320	+	62	NuclAT_1	-	Antitoxin
- (92259)	92259..92320	+	62	NuclAT_1	-	Antitoxin
- (92259)	92259..92320	+	62	NuclAT_1	-	Antitoxin
LO741_RS26110 (92576)	92576..92650	-	75	Protein_89	endonuclease	-
LO741_RS26115 (92896)	92896..93108	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LO741_RS26120 (93245)	93245..93805	-	561	WP_000139315.1	fertility inhibition protein FinO	-
LO741_RS26125 (93908)	93908..94768	-	861	WP_000704513.1	alpha/beta hydrolase	-
LO741_RS26130 (94827)	94827..95573	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..174489	174489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225291 WP_001312851.1 NZ_CP087291:c92215-92066 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225291 NZ_CP121127:c4543822-4542905 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT225291 NZ_CP087291:92259-92320 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin