Detailed information of TA system TA225274

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4651466..4651837
• Replicon: chromosome
• Accession: NZ_CP087290
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A1Y2ZGM7
• Locus tag: LO741_RS22825
• Protein ID: WP_042038331.1
• Coordinates: 4651643..4651837 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4651466..4651644 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS22795 (4647218)	4647218..4647391	+	174	WP_001296046.1	protein YnaL	-
LO741_RS22800 (4647421)	4647421..4648794	+	1374	WP_001272254.1	ATP-dependent RNA helicase DbpA	-
LO741_RS22805 (4648923)	4648923..4649858	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LO741_RS22810 (4649910)	4649910..4651145	-	1236	WP_074413606.1	site-specific integrase	-
LO741_RS22815 (4651147)	4651147..4651362	-	216	WP_000079604.1	excisionase XisR	-
- (4651466)	4651466..4651644	+	179	NuclAT_0	-	Antitoxin
- (4651466)	4651466..4651644	+	179	NuclAT_0	-	Antitoxin
- (4651466)	4651466..4651644	+	179	NuclAT_0	-	Antitoxin
- (4651466)	4651466..4651644	+	179	NuclAT_0	-	Antitoxin
LO741_RS22820 (4651462)	4651462..4651650	-	189	WP_001302840.1	DUF1187 family protein	-
LO741_RS22825 (4651643)	4651643..4651837	-	195	WP_042038331.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LO741_RS22830 (4651894)	4651894..4652703	-	810	WP_065203371.1	recombination protein RecT	-
LO741_RS22835 (4652696)	4652696..4655296	-	2601	WP_065203372.1	exodeoxyribonuclease VIII	-
LO741_RS22840 (4655398)	4655398..4655674	-	277	Protein_4454	hypothetical protein	-
LO741_RS22845 (4655749)	4655749..4655919	-	171	WP_001352098.1	YdaE family protein	-
LO741_RS22850 (4655919)	4655919..4656140	-	222	WP_000560217.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.94 Da
• Isoelectric Point: 8.9538

>T225274 WP_042038331.1 NZ_CP087290:c4651837-4651643 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T225274 NZ_CP121108:c2603479-2603147 [Pantoea sp. X85]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCCGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCATTTAACACGCTCACCCGGTTGCCCGTTGTTGTCCCTGTGACCAGCGGCGGAAACTTTGCTCGCG
AAGCCGGTTTCACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTGATCCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGAAATGGTAAGCGACTGGAGCGTATACCCGACGCTGTCGTGAATGAGGTGCTGGCTCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT225274 NZ_CP087290:4651466-4651644 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1Y2ZGM7

Antitoxin