Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3743539..3744218 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LO741_RS18150 Protein ID WP_000057523.1
Coordinates 3743539..3743841 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LO741_RS18155 Protein ID WP_000806442.1
Coordinates 3743877..3744218 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS18130 (3739109) 3739109..3740693 + 1585 Protein_3536 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LO741_RS18135 (3740730) 3740730..3741209 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LO741_RS18140 (3741437) 3741437..3742417 - 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -
LO741_RS18145 (3742612) 3742612..3743406 - 795 WP_000365161.1 TraB/GumN family protein -
LO741_RS18150 (3743539) 3743539..3743841 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LO741_RS18155 (3743877) 3743877..3744218 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LO741_RS18160 (3744276) 3744276..3746780 - 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
LO741_RS18165 (3747043) 3747043..3747975 + 933 WP_000883024.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3743539..3753622 10083
- flank IS/Tn - - 3741437..3742417 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T225271 WP_000057523.1 NZ_CP087290:3743539-3743841 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T225271 NZ_CP121108:484353-484652 [Pantoea sp. X85]
ATGGTTGAAGATGTGCTGTCGAGTCTCGAACTGCTGAAGGTGTTTGGCCCAATGTTGGGGCGACCGGATGTCGATACCGT
CACCGGCTCTCGTTTTGCCAACATGAAGGAGCTTCGCGTTCAAAGCAATGGAAGGCCGATTCGCGCATTTTTCGCGTTTG
ATCCCGTGAGGAAGGCTATCGTGCTGTGTGCGGGGGATAAAACTGGCGTGAATCAGAAGCGTTTCTACCAATCGATGATC
AAACTAGCTGATAAAGAGTACAAGCAGCATCTGGAGGAATTAACCCATGCCAAAACTTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT225271 WP_000806442.1 NZ_CP087290:3743877-3744218 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT225271 NZ_CP121108:484639-484938 [Pantoea sp. X85]
ATGCCAAAACTTGAAGAACTGATTGCGCAGCGTTCCCCTGAATCTCAGGAGCGTATTAAAAATAAGGCCGACCAAAAAAT
CATGGAAATCCGCCTGCGTCAGTTGCGTGAGGAGCTGGAACTTTCACAGGTTCAGCTGGCTGAAAAGATGGGTATCTCGC
AACCTGCGATCGCGGCGATTGAGGCGCGAGGTGACGAGATAAAACTTTCCAGCCTGAAAAAGTATGTCGAGGCGTTAGGC
GGTAAGCTGAGTCTGAATGTTGAGCTTCCCACCGGAGATGGTCGGATATTTCAGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References