Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2475608..2476210 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag LO741_RS11945 Protein ID WP_000897305.1
Coordinates 2475608..2475919 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LO741_RS11950 Protein ID WP_000356397.1
Coordinates 2475920..2476210 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS11920 (2471522) 2471522..2472121 + 600 WP_001315111.1 glucose-1-phosphatase -
LO741_RS11925 (2472115) 2472115..2472987 + 873 WP_000920762.1 virulence factor BrkB family protein -
LO741_RS11930 (2472984) 2472984..2473421 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
LO741_RS11935 (2473466) 2473466..2474407 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
LO741_RS11940 (2474471) 2474471..2475379 - 909 WP_001318161.1 alpha/beta hydrolase -
LO741_RS11945 (2475608) 2475608..2475919 + 312 WP_000897305.1 hypothetical protein Toxin
LO741_RS11950 (2475920) 2475920..2476210 + 291 WP_000356397.1 NadS family protein Antitoxin
LO741_RS11955 (2476815) 2476815..2477033 + 219 WP_001295676.1 CopG family transcriptional regulator -
LO741_RS11960 (2477248) 2477248..2478177 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
LO741_RS11965 (2478174) 2478174..2478809 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LO741_RS11970 (2478806) 2478806..2479708 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T225265 WP_000897305.1 NZ_CP087290:2475608-2475919 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T225265 NZ_CP121088:c79419-79270 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT225265 WP_000356397.1 NZ_CP087290:2475920-2476210 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT225265 NZ_CP121088:79463-79524 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References