Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1646720..1647519 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LO741_RS07900 Protein ID WP_000347251.1
Coordinates 1647055..1647519 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag LO741_RS07895 Protein ID WP_001296435.1
Coordinates 1646720..1647055 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS07880 (1642504) 1642504..1643274 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
LO741_RS07885 (1643290) 1643290..1644624 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
LO741_RS07890 (1645000) 1645000..1646571 + 1572 WP_001273941.1 galactarate dehydratase -
LO741_RS07895 (1646720) 1646720..1647055 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LO741_RS07900 (1647055) 1647055..1647519 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LO741_RS07905 (1647574) 1647574..1648383 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LO741_RS07910 (1648632) 1648632..1649912 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
LO741_RS07915 (1649935) 1649935..1650408 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LO741_RS07920 (1650419) 1650419..1651198 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LO741_RS07925 (1651188) 1651188..1652066 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LO741_RS07930 (1652084) 1652084..1652518 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1639023..1647519 8496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T225256 WP_000347251.1 NZ_CP087290:1647055-1647519 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T225256 NZ_CP121087:c2567000-2566710 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT225256 WP_001296435.1 NZ_CP087290:1646720-1647055 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT225256 NZ_CP121087:c2567232-2566990 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References